INTRACELLULAR CALCIUM VARIATIONS EVOKED BY MECHANICAL STIMULATION OF MAMMALIAN ISOLATED VESTIBULAR TYPE-I HAIR-CELLS

被引：13

作者：

CHABBERT, C

GELEOC, G

LEHOUELLEUR, J

SANS, A

机构：

[1] Laboratoire de Neurophysiologie sensorielle et cellulaire, Université de Montpellier II U254 INSERM, Montpellier Cedex 05, F-34095, place E. Bataillon

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1994年 / 427卷 / 1-2期

关键词：

FURA-2; GUINEA-PIG; TYPE I VESTIBULAR HAIR CELLS; CALCIUM VARIATIONS; MECHANOTRANSDUCTION;

D O I：

10.1007/BF00585956

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The variations of intracellular free calcium concentration ([Ca2+](i)) were recorded on-line from guinea-pig isolated vestibular sensory cells using a fura-2 fast fluorescent photometry system, during mechanical displacements of the hair bundle. Repetitive displacements of the hair bundle towards the kinocilium (posi tive stimulation 7 degrees, 300ms, 2Hz for 10 s), revealed [Ca2+](i) variations detectable only in the cuticular plate. [Ca2+](i) increased from 105 to 145 nM. Single mechanical displacements of the hair bundle (7 degrees, 200 ms, 0.5Hz) evoked increases of [Ca2+](i) from 50 +/- 23 nM to 139 +/- 79 (n = 12). In the opposite direction, the mechanical stimulations (8 degrees, 400 ms, 0.5Hz) evoked a decrease of [Ca2+](i) from 68 +/- 17 nM to 37 +/- 12 nM (n = 8). The variations of [Ca2+](i) detected in the cuticular plate during positive displacements of the hair bundle were reversibly abolished in the presence of 100 mu M gentamicin and they could not be evoked in 0.1 mM calcium in the external medium. From these experiments, it has been concluded that the [Ca2+](i) variations recorded in the cuticular plate were due to a limited entry of calcium ions through transduction channels localized in the hair bundle. The typical kinetics of variations of [Ca2+](i) evoked during positive displacements of the hair bundle should account for the presence of strong calcium regulation systems in the hair bundle and cuticular plate.

引用

页码：162 / 168

页数：7

共 35 条

[11]

EATOCK RA, 1987, J NEUROSCI, V7, P2821

[12] DEVELOPMENT OF VESTIBULAR EFFERENT NERVE-ENDINGS DURING CAT MATURATION - ULTRASTRUCTURAL-STUDY [J].

FAVRE, D ;

SANS, A .

BRAIN RESEARCH, 1978, 142 (02) :333-337

[13] INWARD POTASSIUM RECTIFIER CURRENT IN TYPE-I VESTIBULAR HAIR-CELLS ISOLATED FROM GUINEA-PIG [J].

GRIGUER, C ;

SANS, A ;

VALMIER, J ;

LEHOUELLEUR, J .

NEUROSCIENCE LETTERS, 1993, 149 (01) :51-55

[14]

GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440

[15]

HACOHEN N, 1989, J NEUROSCI, V9, P3988

[16] MECHANICAL RELAXATION OF THE HAIR BUNDLE MEDIATES ADAPTATION IN MECHANOELECTRICAL TRANSDUCTION BY THE BULLFROGS SACCULAR HAIR CELL [J].

HOWARD, J ;

HUDSPETH, AJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (09) :3064-3068

[17]

HOWARD J, 1988, ANNU REV BIOPHYS BIO, V17, P99

[18] COMPLIANCE OF THE HAIR BUNDLE ASSOCIATED WITH GATING OF MECHANOELECTRICAL TRANSDUCTION CHANNELS IN THE BULLFROGS SACCULAR HAIR CELL [J].

HOWARD, J ;

HUDSPETH, AJ .

NEURON, 1988, 1 (03) :189-199

[19]

HUANG PL, 1990, BIOPHYS J, V57, P450

[20] SENSITIVITY, POLARITY, AND CONDUCTANCE CHANGE IN RESPONSE OF VERTEBRATE HAIR CELLS TO CONTROLLED MECHANICAL STIMULI [J].

HUDSPETH, AJ ;

COREY, DP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (06) :2407-2411

← 1 2 3 4 →