OVERCOMING INCLUSION BODY FORMATION IN A HIGH-LEVEL EXPRESSION SYSTEM

被引：98

作者：

MOORE, JT ^{[1
]}

UPPAL, A ^{[1
]}

MALEY, F ^{[1
]}

MALEY, GF ^{[1
]}

机构：

[1] SUNY ALBANY,SCH PUBL HLTH,DEPT BIOMED SCI,ALBANY,NY 12201

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1993年 / 4卷 / 02期

关键词：

D O I：

10.1006/prep.1993.1022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Attempts at overexpressing T4-phage deoxycytidylate deaminase using the pET3c/BL21(DE3)/pLysS system resulted in this enzyme being part of an inactive inclusion-body complex. However, by employing an enriched growth medium it was found that the deaminase could be induced in a soluble active form to at least 20% of this organism′s cellular protein. Insoluble inclusion bodies were obtained with less rich media. This procedure was employed successfully with other highly expressed proteins that formed inclusion bodies. The use of a rich growth medium during the course of protein induction may be a valuable adjunct to limiting inclusion body formation with this as well as other expression systems. © 1993 Academic Press. All rights reserved.

引用

页码：160 / 163

页数：4