ABNORMAL TAU PROTEINS FROM ALZHEIMERS-DISEASE BRAINS - PURIFICATION AND AMINO-ACID-ANALYSIS

被引：0

作者：

LIU, WK ^{[1
]}

KSIEZAKREDING, H ^{[1
]}

YEN, SH ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PATHOL,F-538,1300 MORRIS PK AVE,BRONX,NY 10461

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1991年 / 266卷 / 32期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abnormal tau proteins (PHF-tau) were isolated from Alzheimer's disease brains by treatment of paired helical filament enriched-fractions with perchloric acid and boiling of the acid precipitable fraction with beta-mercaptoethanol. These proteins were purified further by a second perchloric acid treatment. The purified PHF-tau proteins were soluble in buffers devoid of sodium dodecyl sulfate. However, they were similar to the abnormal tau extracted from paired helical filaments with sodium dodecyl sulfate, also named A68, in molecular mass (68, 64, and 60 kDa), isoelectric point (pI 5.5-6.5), reactivity with anti-tau antibodies, and in requirement for alkaline phosphatase treatment to bind the Tau-1 antibody. Compared to normal tau, the soluble PHF-tau contained 100% more glycine and 35% less lysine residue. The results suggest that besides phosphorylation other types of modification may be involved in differentiating PHF-tau from normal tau.

引用

页码：21723 / 21727

页数：5

共 38 条

[1] ACCUMULATION OF ABNORMALLY PHOSPHORYLATED-TAU PRECEDES THE FORMATION OF NEUROFIBRILLARY TANGLES IN ALZHEIMERS-DISEASE [J].

BANCHER, C ;

BRUNNER, C ;

LASSMANN, H ;

BUDKA, H ;

JELLINGER, K ;

WICHE, G ;

SEITELBERGER, F ;

GRUNDKEIQBAL, I ;

IQBAL, K ;

WISNIEWSKI, HM .

BRAIN RESEARCH, 1989, 477 (1-2) :90-99

[2]

BAUDIER J, 1987, J BIOL CHEM, V262, P17584

[3] OCCURRENCE OF NEUROPIL THREADS IN THE SENILE HUMAN-BRAIN AND IN ALZHEIMERS-DISEASE - A 3RD LOCATION OF PAIRED HELICAL FILAMENTS OUTSIDE OF NEUROFIBRILLARY TANGLES AND NEURITIC PLAQUES [J].

BRAAK, H ;

BRAAK, E ;

GRUNDKEIQBAL, I ;

IQBAL, K .

NEUROSCIENCE LETTERS, 1986, 65 (03) :351-355

[4] INVITRO CONDITIONS FOR THE SELF-POLYMERIZATION OF THE MICROTUBULE-ASSOCIATED PROTEIN, TAU FACTOR [J].

DEGARCINI, EM ;

AVILA, J .

JOURNAL OF BIOCHEMISTRY, 1987, 102 (06) :1415-1421

[5] ALZHEIMERS-DISEASE - TAU PROTEINS, THE PROMOTING FACTORS OF MICROTUBULE ASSEMBLY, ARE MAJOR COMPONENTS OF PAIRED HELICAL FILAMENTS [J].

DELACOURTE, A ;

DEFOSSEZ, A .

JOURNAL OF THE NEUROLOGICAL SCIENCES, 1986, 76 (2-3) :173-186

[6] POSTTRANSLATIONAL GLUTAMYLATION OF ALPHA-TUBULIN [J].

EDDE, B ;

ROSSIER, J ;

LECAER, JP ;

DESBRUYERES, E ;

GROS, F ;

DENOULET, P .

SCIENCE, 1990, 247 (4938) :83-85

[7]

FLAMENT S, 1989, CR ACAD SCI III-VIE, V308, P77

[8] PROTEIN-TAU VARIANTS PRESENT IN PAIRED HELICAL FILAMENTS (PHFS) OF ALZHEIMER BRAINS [J].

GACHE, Y ;

RICOLFI, F ;

GUILLEMINOT, J ;

THEISS, G ;

NUNEZ, J .

FEBS LETTERS, 1990, 272 (1-2) :65-68

[9] MULTIPLE ISOFORMS OF HUMAN MICROTUBULE-ASSOCIATED PROTEIN-TAU - SEQUENCES AND LOCALIZATION IN NEUROFIBRILLARY TANGLES OF ALZHEIMERS-DISEASE [J].

GOEDERT, M ;

SPILLANTINI, MG ;

JAKES, R ;

RUTHERFORD, D ;

CROWTHER, RA .

NEURON, 1989, 3 (04) :519-526

[10] A PREPARATION OF ALZHEIMER PAIRED HELICAL FILAMENTS THAT DISPLAYS DISTINCT TAU-PROTEINS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

GREENBERG, SG ;

DAVIES, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) :5827-5831

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