CLASS-I (H-2KB) GENE TRANSFECTION REDUCES SUSCEPTIBILITY OF YAC-1 LYMPHOMA TARGETS TO NATURAL-KILLER-CELLS

被引：18

作者：

CARLOW, DA ^{[1
]}

PAYNE, U ^{[1
]}

HOZUMI, N ^{[1
]}

RODER, JC ^{[1
]}

CZITROM, AA ^{[1
]}

机构：

[1] UNIV TORONTO,MT SINAI HOSP,RES INST,600 UNIV AVE,SUITE 476,TORONTO M5G 1X5,ONTARIO,CANADA

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1990年 / 20卷 / 04期

关键词：

D O I：

10.1002/eji.1830200419

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A “hybrid gene” (MTKb) comprised of the human metallothionein IIA promoter ligated to the genomic sequence of the major histocompatibility complex class I (H‐2Kb) gene was subcloned into the expression vector pSV2neo and transfected into the natural killer (NK) cell‐sensitive YAC‐1 lymphoma. The Kb gene product was readily detectable on the cell surface of G418‐resistant transfectants using both Kb‐specific monoclonal antibodies and H‐2b‐specific cytolytic T cells. Unlike control pSV2neo transfectants, MTKb‐pSV2neo transfectants were relatively resistant to lysis by NK cells from H‐2a, H‐2b, H‐2k or H‐2 (a × b)F1 haplotype mice. These data strongly suggest that the effects of MHC expression on susceptibility to NK cells can be mediated by a single and well‐defined class I molecule, Kb. Copyright © 1990 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：841 / 846

页数：6

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