IDENTIFICATION OF STRUCTURAL NITROGEN-FIXATION (NIF) GENES IN BACILLUS-POLYMYXA AND BACILLUS-MACERANS

被引：7

作者：

OLIVEIRA, SS ^{[1
]}

SELDIN, L ^{[1
]}

BASTOS, MCF ^{[1
]}

机构：

[1] UNIV FED RIO JANEIRO,CCS,INST MICROBIOL,DEPT MICROBIOL GERAL,CIDADE UNIV,BR-21944970 RIO JANEIRO,BRAZIL

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1993年 / 9卷 / 03期

关键词：

BACILLUS-MACERANS; BACILLUS-POLYMYXA; NIF GENES; NITROGEN FIXATION;

D O I：

10.1007/BF00383088

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Some naturally occurring Nif+ and Nif- strains of Bacillus polymyxa and Bacillus macerans were tested for DNA homology to the Klebsiella pneumoniae nif genes by Southern hybridization. Genomic DNA from all Nif+ strains carried homologous sequences only to structural nif genes. The homology detected was limited to nifH and niFD. The hybridization pattern observed suggested that both genes are contiguous on the chromosome of the Bacillus strains. No homology was found between the genomic DNA from Nif- strains and the K. pneumoniae nif genes.

引用

页码：387 / 389

页数：3

共 6 条

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[3] Seldin L., Bastos M.C.F., Penido E.G.C., Identification of Bacillus azotofixans nitrogen fixation genes using heterologous nif probes, Nitrogen Fixation with Non Legumes, pp. 179-187, (1989)
[4] Seldin L., Van Elsas J.D., Penido E.G.C., Bacillus nitrogen fixers from Brazilian soils, Plant and Soil, 70, pp. 243-255, (1983)
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