A METHOD FOR RAPID-DETERMINATION OF LORAZEPAM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：18
|
作者：
KONDO, T [1 ]
BUSS, DC [1 ]
ROUTLEDGE, PA [1 ]
机构：
[1] LLANDOUGH HOSP,DEPT CLIN PHARMACOL,CARDIFF,WALES
来源：
THERAPEUTIC DRUG MONITORING | 1993年 / 15卷 / 01期
关键词：
LORAZEPAM; SOLID-PHASE EXTRACTION; REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;
D O I：
10.1097/00007691-199302000-00006
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
A new sensitive method using solid-phase column extraction and high-performance liquid chromatography (HPLC) has been developed for the determination of lorazepam and its glucuronide metabolite in human plasma. Extraction was performed with a C2-Bond Elut column followed by reverse-phase HPLC of the sample. The detection limit of lorazepam in plasma was 0.5 mug/L. Standard curves over the concentration range from 2.5 to 75 mug/L had good linearity. Intra-assay variability ranged from 2.0 to 6.2% and interassay variability from 4.8 to 8% at the concentration range of 5-50 mug/L.
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页码：35 / 38
页数：4
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