COMPARISON OF A NOVEL THIN-LAYER CHROMATOGRAPHIC-FLUORESCENCE DETECTION METHOD WITH A SPECTROFLUOROMETRIC METHOD FOR THE DETERMINATION OF 7-HYDROXYCOUMARIN IN HUMAN URINE

被引：73

作者：

CHOLERTON, S ^{[1
]}

IDLE, ME ^{[1
]}

VAS, A ^{[1
]}

GONZALEZ, FJ ^{[1
]}

IDLE, JR ^{[1
]}

机构：

[1] NIH,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 575卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0378-4347(92)80166-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method for the determination of 7-hydroxycoumarin in human urine which combines thin-layer chromatography (TLC) with fluorescence detection (FD) has been devised. The limit of detection (I ng/ml) enables determination of 7-hydroxycoumarin after both administration of coumarin and environmental exposure to this fragrance material. When compared to a spectrofluorometric method of analysis, the TLC-FD method proved to be more selective for the analysis of 7-hydroxycoumarin in human urine.

引用

页码：325 / 330

页数：6

共 10 条

[1] CRITICAL-REVIEW OF THE TOXICOLOGY OF COUMARIN WITH SPECIAL REFERENCE TO INTERSPECIES DIFFERENCES IN METABOLISM AND HEPATOTOXIC RESPONSE AND THEIR SIGNIFICANCE TO MAN [J].