HEAT-SHOCK INDUCES ENZYMES OF TREHALOSE METABOLISM, TREHALOSE ACCUMULATION, AND THERMOTOLERANCE IN SCHIZOSACCHAROMYCES-POMBE, EVEN IN THE PRESENCE OF CYCLOHEXIMIDE

被引：100

作者：

DEVIRGILIO, C ^{[1
]}

SIMMEN, U ^{[1
]}

HOTTIGER, T ^{[1
]}

BOLLER, T ^{[1
]}

WIEMKEN, A ^{[1
]}

机构：

[1] UNIV BASEL,DEPT BOT,CH-4051 BASEL,SWITZERLAND

来源：

FEBS LETTERS | 1990年 / 273卷 / 1-2期

关键词：

Heat shock; Schizosaccharomyces pombe; Thermotolerance; Trehalase; Trehalose; Trehalose-6-phosphate synthase;

D O I：

10.1016/0014-5793(90)81062-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exponentially growing cells of the fission yeast, Schizosaccharomyces pombe, contained virtually no trehalose at 27°C but rapidly accumulated large quantities during heat shock at 40°C. Activities of trehalose-6-phosphate synthase and trehalase also increased upon heat shock. Thermotolerance of the cells, measured as survival at 52°C, increased in parallel to trehalose accumulation and decreased in parallel to the trehalose levels when cells were shifted back to 27°C. Trehalose levels, activities of enzymes of trehalose metabolism and thermotolerance strongly increased upon heat shock even in the presence of cycloheximide, indicating that none of these effects requires protein synthesis. The data support the hypothesis that trehalose acts as a thermoprotectant in Schizosaccharomyces pombe. © 1990.

引用

页码：107 / 110

页数：4

共 17 条

[1] PRESERVATION OF MEMBRANES IN ANHYDROBIOTIC ORGANISMS - THE ROLE OF TREHALOSE [J].