Plant Assays for Quantifying Ralstonia solanacearum Virulence

被引:24
|
作者
Khokhani, Devanshi [1 ]
Tuan Minh Tran [2 ]
Lowe-Power, Tiffany M. [3 ]
Allen, Caitilyn [4 ]
机构
[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
[2] Nanyang Technol Univ, Sch Biol Sci, Singapore, Singapore
[3] Univ Calif Berkeley, Plant & Microbial Biol, Berkeley, CA 94720 USA
[4] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA
来源
BIO-PROTOCOL | 2018年 / 8卷 / 18期
关键词
Virulence assay; Bacterial wilt; Vascular plant pathogen; Plant colonization; Xylem sap; R; solanacearum; Tomato;
D O I
10.21769/BioProtoc.3028
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Virulence assays are powerful tools to study microbial pathogenesis in vivo. Good assays track disease development and, coupled with targeted mutagenesis, can identify pathogen virulence factors. Disease development in plants is extremely sensitive to environmental factors such as temperature, atmospheric humidity, and soil water level, so it can be challenging to standardize conditions to achieve consistent results. Here, we present optimized and validated experimental conditions and analysis methods for nine assays that measure specific aspects of virulence in the phytopathogenic bacterium Ralstonia solanacearum, using tomato as the model host plant.
引用
收藏
页数:19
相关论文
共 50 条
  • [41] The effector protein RipBJ of Ralstonia solanacearum elicits plant immunity
    Cheng, C. P.
    Chang, C. H.
    Chu, L. W.
    Chu, T. H.
    Li, C. H.
    Liao, P. S.
    Wu, C. S.
    MOLECULAR PLANT-MICROBE INTERACTIONS, 2024, 37 (05) : 21 - 21
  • [42] Novel plant inputs influencing Ralstonia solanacearum during infection
    Paola Zuluaga, A.
    Puigvert, Marina
    Valls, Marc
    FRONTIERS IN MICROBIOLOGY, 2013, 4
  • [43] Proteomic comparison of Ralstonia solanacearum strains reveals temperature dependent virulence factors
    Ana M Bocsanczy
    Ute CM Achenbach
    Arianna Mangravita-Novo
    Marjorie Chow
    David J Norman
    BMC Genomics, 15
  • [44] Correction to: Ralstonia solanacearum virulence in eggplant seedlings by the leaf-clip inoculation
    Tarinee Phukan
    Kristi Kabyashree
    Radhika Singh
    Pankaj L. Sharma
    Niraj Singh
    Anjan Barman
    Biswa R. Jena
    Suvendra K. Ray
    Phytopathology Research, 1
  • [45] Comparative Genomics of Ralstonia solanacearum Identifies Candidate Genes Associated with Cool Virulence
    Bocsanczy, Ana M.
    Huguet-Tapia, Jose C.
    Norman, David J.
    FRONTIERS IN PLANT SCIENCE, 2017, 8
  • [46] Extracellular DNases of Ralstonia solanacearum modulate biofilms and facilitate bacterial wilt virulence
    Tuan Minh Tran
    MacIntyre, April
    Khokhani, Devanshi
    Hawes, Martha
    Allen, Caitilyn
    ENVIRONMENTAL MICROBIOLOGY, 2016, 18 (11) : 4103 - 4117
  • [47] Loss of Virulence of the Phytopathogen Ralstonia solanacearum Through Infection by φφRSM Filamentous Phages
    Addy, Hardian S.
    Askora, Ahmed
    Kawasaki, Takeru
    Fujie, Makoto
    Yamada, Takashi
    PHYTOPATHOLOGY, 2012, 102 (05) : 469 - 477
  • [48] Ralstonia solanacearum AmpD is required for wild-type bacterial wilt virulence
    Tans-Kersten, J.
    Gay, J.
    Allen, C.
    MOLECULAR PLANT PATHOLOGY, 2000, 1 (03) : 179 - 185
  • [49] Using Proteomics and Genomics to Search for Low Temperature Virulence Factors in Ralstonia solanacearum
    Bocsanczy, A. M.
    Norman, D. J.
    Huguet-Tapia, J. C.
    Achenbach, U.
    Mangravita-Novo, A.
    IV INTERNATIONAL SYMPOSIUM ON TOMATO DISEASES, 2015, 1069 : 33 - 37
  • [50] The inhibitory effects of different types of Brassica seed meals on the virulence of Ralstonia solanacearum
    Peng, Junwei
    Liu, Hong
    Shen, Minchong
    Chen, Ruihuan
    Li, Jiangang
    Dong, Yuanhua
    PEST MANAGEMENT SCIENCE, 2021, 77 (11) : 5129 - 5138