SUBCELLULAR-LOCALIZATION OF ACIDIC AND BASIC PR PROTEINS IN TOBACCO MOSAIC-VIRUS-INFECTED TOBACCO

被引:29
作者
DORE, I
LEGRAND, M
CORNELISSEN, BJC
BOL, JF
机构
[1] CNRS,INST BIOL MOLEC PLANTES,STRASBOURG,FRANCE
[2] MOGEN INT,LEIDEN,NETHERLANDS
关键词
D O I
10.1007/BF01310952
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Infection of Samsum NN tobacco with tobacco mosaic virus (TMV) results in the induction of the synthesis of acidic and basic isoforms of many pathogenesis-related (PR) proteins. By immunogold-electronmicroscopy we have shown that PR proteins accumulate mainly in cells around the necrotic spots of TMV-induced lesions. The acidic chitinases, beta-(1,3)-glucanases and thaumatin-like proteins were found to accumulate in extracellular "pocket-like" vesicles while the basic chitinases were found in electron dense inclusion bodies in the vacuoles. These structures were not detectable in PR-containing leaves devoid of virus nor in healthy plants.
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页码:97 / 107
页数:11
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