CAAT ENHANCER BINDING-PROTEIN IS ABLE TO BIND TO ATF CRE ELEMENTS

被引：55

作者：

BAKKER, O ^{[1
]}

PARKER, MG ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,MOLEC ENDOCRINOL LAB,LINCOLNS INN FIELDS,LONDON WC2A 3PX,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 06期

关键词：

D O I：

10.1093/nar/19.6.1213

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcription factor C/EBP is known to be able to bind to two different classes of sites, a CAAT-box and one present in a number of viral enhancers. In this paper we show using band-shift assays, methylation interference and footprinting that C/EBP is also able to bind with high affinity to ATF/CRE sites. Competition with mutant ATF sites and methylation interference indicate that C/EBP may be able to bind to the ATF/CRE sites by virtue of their homology to the enhancer core elements. Furthermore, we show that C/EBP is able to direct transcription from this site in transient transfection experiments and that mutations in the ATF binding site that impair DNA binding also effect the ability of C/EBP to stimulate transcription.

引用

页码：1213 / 1217

页数：5

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