Effects of molecular hydrogen-dissolved alkaline electrolyzed water on intestinal environment in mice

被引:33
作者
Higashimura, Yasuki [1 ,2 ]
Baba, Yasunori [3 ]
Inoue, Ryo [4 ]
Takagi, Tomohisa [2 ]
Uchiyama, Kazuhiko [2 ]
Mizushima, Katsura [2 ]
Hirai, Yasuko [2 ]
Ushiroda, Chihiro [2 ]
Tanaka, Yoshinori [5 ]
Naito, Yuji [2 ]
机构
[1] Ishikawa Prefectural Univ, Dept Food Sci, Nonoichi, Ishikawa, Japan
[2] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Mol Gastroenterol & Hepatol, Kyoto, Japan
[3] Ishikawa Prefectural Univ, Res Inst Bioresources & Biotechnol, Nonoichi, Ishikawa, Japan
[4] Kyoto Prefectural Univ, Dept Agr & Life Sci, Lab Anim Sci, Kyoto, Japan
[5] Panasonic Corp, Appliances Co, Kusatsu, Shiga, Japan
基金
日本学术振兴会;
关键词
gut microbiota; propionic acid; low-density lipoprotein cholesterol; alanine aminotransferase; hydrogen; alkaline electrolyzed water; 16S rRNA; Clostridiaceae;
D O I
10.4103/2045-9912.229597
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Increasing evidence indicates that molecular hydrogen-dissolved alkaline electrolyzed water (AEW) has various physiological activities such as antioxidative activity. Gut microbiota are deeply associated with our health through a symbiotic relationship. Recent reports have described that most gastrointestinal microbial species encode the genetic capacity to metabolize molecular hydrogen, meaning that molecular hydrogen might affect the gut microbial composition. Nevertheless, AEW effects on gut microbiota remain unknown. This study investigated AEW effects on the intestinal environment in mice, including microbial composition and short-chain fatty acid contents. After mice were administered AEW for 4 weeks, 16S rRNA gene sequencing analyses revealed their fecal microbiota profiles. Organic acid concentrations in cecal contents were measured using an HPLC system. Compared to the control group, AEW administration mice had significantly lower serum low-density lipoprotein cholesterol level and alanine aminotransferase activity. Organic acid concentrations of propionic, isobutyric, and isovaleric acids were higher in AEW-administered mice. Results of 16S rRNA gene sequencing analyses showed that the relative abundances of 20 taxa differed significantly in AEW-administered mice. Although the definitive role of gut microbes of AEW-administered mice remains unknown, our data demonstrate the possibility that AEW administration affects the gut microbial composition and that it has beneficial health effects in terms of cholesterol metabolism and liver protection.
引用
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页码:6 / +
页数:7
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