DETERMINATION OF PROCAINE PENICILLIN-G AND PENICILLIN-G BY 2ND DERIVATIVE SPECTROPHOTOMETRY IN PHARMACEUTICAL PRODUCTS

''Zero-crossing'' derivative spectrophotometry has been used for determining binary mixtures of penicillin G and procaine penicillin G. The procedure is rapid, simple, nondestructive, and does not require resolutions of equations. Calibration graphs are linear between 2.0 and 50.0 mug mL-1 of the penicillin G at 226.0 nm, and between 2.0 and 100.0 mug mL-1 of procaine penicillin G at 319.0 nm, in the presence of each other. A complete and exhaustive statistical analysis of the experimental data was realized to demonstrate the validity of method. The method was successfully applied to assay commercial injections of these drugs.