STIMULATION OF NA+/H+ EXCHANGE INDUCED BY ANGIOTENSIN-II IN CULTURED RAT VASCULAR SMOOTH-MUSCLE CELLS - ROLE OF CA2+ AND C-KINASE

Changes in cytosolic calcium concentration ([Ca2+]i) have been implicated in the regulation of intracellular pH (pHi) in several cell types. In the present study we investigated the regulatory mechanism of Na+/H+ exchange induced by angiotensin II (AII) in cultured rat vascular smooth muscle cells (VSMCs). Serially passaged VSMCs from Sprague-Dawley rat thoracic aorta were grown on coverslips and loaded with the pH-sensitive fluorescent indicator 2',7'-bis (carboxyethyl)-5,6-carboxyfluorescein (BCECF). In HCO3--free Ringer solution, pH 7.40, the resting pHi was 7.21 +/- 0.01 (n = 21). A biphasic response was seen after exposure of these cells to AII: an initial transient a acidification, followed by sustained alkalization. The magnitude of alkalization was dose-dependent. AII-mediated acidification was completely inhibited by [Sar1-IIe5-Gly8]AII, but amiloride had no effect. In contrast, the alkalization induced by AII was abolished by both amiloride and Na+-free medium. In Ca2+-free medium, the AII-induced alkalization was partially blocked and verapamil also caused partial inhibition. Since AII activates phospholipase C in VSMCs, we examined whether All would increase Na+/H+ exchange by activation of protein kinase C. An inhibitor of protein kinase C, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), partially inhibited the alkalization induced by AII. These results indicate that AII stimulates cytoplasmic alkalization via an amiloride-sensitive Na+/H+ exchange system in cultured rat VSMCs, and that this AII-stimulated Na+/H+ exchange is mediated by Ca2+-dependent and protein kinase C-dependent mechanisms.