ELECTRON-MICROSCOPY OF HYBRIDOMA CELLS WITH SPECIAL REGARD TO MONOCLONAL-ANTIBODY PRODUCTION

被引：52

作者：

ALRUBEAI, M

MILLS, D

EMERY, AN

机构：

[1] School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT

[2] School of Biochemistry, University of Birmingham, Birmingham, B15 2TT

来源：

CYTOTECHNOLOGY | 1990年 / 4卷 / 01期

关键词：

electron microscopy; endoplasmic reticulum; hybridoma; monoclonal antibody;

D O I：

10.1007/BF00148807

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Electron microscopy of mouse hybridoma cell lines shows that the major difference between non, low and high producer cell lines is the amount of endoplasmic reticulum. Vesicular-tubular or cavernous structures of endoplasmic reticulum, which can survive long after cell death, are particularly abundant in producer cell lines. Immunogold labelling with anti-mouse IgG reveals that antibodies are predominantly located in these structures. The cell membrane undergoes structural changes during the late stages of batch culture with the disappearance of microvilli and the appearance of blebs and deep indentations. Necrosis disrupts the cytoplasmic structures and the nucleus is last to degrade. © 1990 Kluwer Academic Publishers.

引用

页码：13 / 28

页数：16

共 7 条

[1]

Al-Rubeai M.A., Rookes S., Emery A.N., Flow cytometric studies during synchronous and asynchronous suspension cultures of hybridoma cells, Advances in Animal Cell Biology and Technology for Bioprocesses, pp. 241-245, (1989)

[2]

Biberfield P., Uropod formation in phytohaemagglutinin (PHA) stimulated lymphocytes, Experimental Cell Research, 66, pp. 433-445, (1971)

[3]

Bienkowski R.S., Intracellular degradation of newly synthesised secretory proteins, Biochem. J., 214, pp. 1-10, (1983)

[4]

Doorbar J., Evans H.S., Coneron J., Crawford L.V., Gallimore P.H., Analysis of HPV-1 E4 gene expression using epitope defined antibodies, EMBO J., 7, pp. 825-832, (1988)

[5]

Sheldrake A.R., The ageing, growth and death of cells, Nature, 250, pp. 381-385, (1974)

[6]

Wyllie A.H., Cell death: a new classification separating apoptosis from necrosis, Cell death in biology and pathology, pp. 9-34, (1981)

[7]

Wyllie A.H., Duvall E., Blow J.J., Intracellular mechanisms in cell death in normal and pathological tissues, Cell Ageing and Cell Death, pp. 269-294, (1984)

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