THERE ARE 2 MAJOR TYPES OF HEPATITIS-C VIRUS IN JAPAN

被引：317
作者：
ENOMOTO, N
TAKADA, A
NAKAO, T
DATE, T
机构：
[1] KANAZAWA MED UNIV,MED RES INST,DIV CANC RES,UCHINADA CHO,KAHOKU,ISHIKAWA 92002,JAPAN
[2] KANAZAWA MED UNIV,DEPT INTERNAL MED,DIV GASTROENTEROL,KAHOKU,ISHIKAWA 92002,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 03期
关键词：
D O I：
10.1016/0006-291X(90)90494-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The polymerase chain reaction (PCR) was used to detect hepatitis C virus (HCV) in plasma from chronic non-A, non-B hepatitis patients. By choice of adequate primers, 19 of 24 samples (79%) were found positive. Sequence analysis of amplified 400 bp cDNA fragments encoding a portion of NS5 gene suggested that HCV can be classified into two types (named K1 and K2) in Japan. Slot blot hybridization of the fragments indicated that 13 were HCV-K1 and 6 were HCV-K2, which show 80% and 67% nucleotide sequence homology, respectively, with that of the prototype. © 1990.
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页码：1021 / 1025
页数：5
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