Analysis of extracellular RNA by digital PCR

被引：30

作者：

Takahashi, Kenji ^{[1
]}

Yan, Irene K. ^{[1
]}

Kim, Chaeyoung ^{[2
]}

Kim, Jungsu ^{[2
]}

Patel, Tushar ^{[1
]}

机构：

[1] Mayo Clin, Dept Transplantat & Canc Biol, 4500 San Pablo Rd, Jacksonville, FL 32224 USA

[2] Mayo Clin, Dept Neurosci, Jacksonville, FL 32224 USA

来源：

FRONTIERS IN ONCOLOGY | 2014年 / 4卷

关键词：

non-coding RNA; exosomes; extracellular vesicles; gene expression; detection; real-time PCR;

D O I：

10.3389/fonc.2014.00129

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The transfer of extracellular RNA is emerging as an important mechanism for inter-cellular communication. The ability for the transfer of functionally active RNA molecules from one cell to another within vesicles such as exosomes enables a cell to modulate cellular signaling and biological processes within recipient cells. The study of extracellular RNA requires sensitive methods for the detection of these molecules. In this methods article, we will describe protocols for the detection of such extracellular RNA using sensitive detection technologies such as digital PCR. These protocols should be valuable to researchers interested in the role and contribution of extracellular RNA to tumor cell biology.

引用

页数：6

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