Association of of IL-1 receptor antagonist (IL-1RN) and interleukin-1 beta genes (IL-1 beta) polymorphisms with recurrent pregnancy loss in Iranian Azeri women

Objective: One of the most important problems in human reproduction is recurrent pregnancy loss (RPL). RPL is defined as three or more consecutive abortions in the first trimester of pregnancy. The association between the polymorphisms in the immunological factors and RPL was investigated. The aim of our study was to determine the association of interleukin receptor antagonist (IL-IRN) and interleukin-1 beta (IL-1 beta) polymorphisms with RPL in Iranian Azeri women. Materials and methods: The study participants consisted of 100 women with RPL of Iranian Azeri origin. The control group comprised 100 age-and ethnically-matched healthy women of the same reproductive age. Genomic DNA was extracted from the whole blood and genotype determinations were performed using polymerase chain reaction (PCR) amplification followed by restriction fragment length polymorphism (RFLP) analysis. Results: Our results showed no significant relationship between IL-1RN polymorphism and RPL. The homozygous state in -857 C/T variant was seen to be higher in RPL patients than in control subjects. Also frequency of wild type genotype was lower in RPL patients than in controls. However, this associations was not significant. Conclusion: This study suggested that -511 C/T (rs16944) and -31 C/T (rs1143627) polymorphisms in IL-1 beta gene may not be involved in RPL in Iranian Azeri women. Also the promoter polymorphism of the IL-1RN gene may not play a role in the susceptibility to RPL.