PLASMODIUM-FALCIPARUM - A METHOD FOR THE AMPLIFICATION OF S-ANTIGENS AND ITS APPLICATION TO LABORATORY AND FIELD SAMPLES

被引：17
作者：
KYES, S [1 ]
CRAIG, AG [1 ]
MARSH, K [1 ]
NEWBOLD, CI [1 ]
机构：
[1] KEMRI, COASTAL UNIT, KILIFI, KENYA
来源：
EXPERIMENTAL PARASITOLOGY | 1993年 / 77卷 / 04期
基金：
英国惠康基金;
关键词：
S-ANTIGEN; MALARIA; POLYMORPHISM; STRAIN IDENTIFICATION;
D O I：
10.1006/expr.1993.1108
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates. © 1993 by Academic Press, Inc.
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收藏
页码：473 / 483
页数：11
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