Determination of Cefaclor in Human Plasma by Reversed-Phase High-Performance Liquid Chromatography with UV Detection and Its Application to the Bioequivalence Studies

被引：3

作者：

Jiang, Bo ^{[1
]}

Ruan, Zourong ^{[1
]}

Lou, Honggang ^{[1
]}

Xu, Donghang ^{[1
]}

Yuan, Hong ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Div Clin Pharmacol, Affiliated Hosp 2, Hangzhou 310009, Zhejiang, Peoples R China

来源：

ANALYTICAL LETTERS | 2009年 / 42卷 / 14期

关键词：

Cefaclor; HPLC; human plasma;

D O I：

10.1080/00032710903137467

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A selective and sensitive reversed-phase high-performance liquid chromatography method was developed and validated for quantitation of cefaclor in human plasma using cefradine as an internal standard. Calibration curve was linear over range of 0.1-20 mg . L-1. The intra- and inter-run relative standard deviations of the assay were less than 7%. The mean absolute recoveries determined at the concentrations of 0.3, 3.0, 8.0, and 15.0 mg . L-1 were 69.9%, 69.9%, 77.1% and 72.0%, respectively. The analytical method established was proved to be specific, precise, sensitive, and suitable for applying in the pharmacokinetic and bioequivalence studies of cefaclor in human.

引用

页码：2170 / 2179

页数：10

共 12 条

[1] Determination of cefaclor in human plasma by a sensitive and specific liquid chromatographic-tandem mass spectrometric method [J].