ANTIMICROBIAL, CYTOTOXIC AND ANTIOXIDANT ACTIVITY OF THE EXUDATE OF CALOTROPIS GIGANTEA

Plant is one of the important source of drugs that's why we have done this project work to find out some biological activities of the plant exudates of Calotropis gigantea where antimicrobial, cytotoxic and antioxidant activity were analyzed. To test of antimicrobial activity, disc diffusion method has been followed. The exudate of the plant has shown antimicrobial activity against four microorganisms such as Escherichia coli (15mm), Vibrio mimicus (15mm), Vibrio parahemolytics (15mm) and Staphylococcus aureus (9mm). The exudates of the plant has shown cytotoxic activity which was done by following the brine shrimp lethality bioassay method. The result of the test has demonstrated good cytotoxic activity against Brine Shrimp Nauplii with a LC50 value of 12.66 mu g/ml. The exudate of the plant has demonstrated good antioxidant activity with a LC50 value of 3.301 mu g/ml.. So from the project we can say that further investigation should be carried out to get the active principles.