EFFICIENT ANALYSIS OF CYTOCHROME P4501A CATALYTIC ACTIVITY, PORPHYRINS, AND TOTAL PROTEINS IN CHICKEN-EMBRYO HEPATOCYTE CULTURES WITH A FLUORESCENCE PLATE READER

被引：98
作者：
KENNEDY, SW
JONES, SP
BASTIEN, LJ
机构：
[1] Environm Canada, Canadian Wildlife Serv, Hull, Que. K1A 0H3, Natl Wildlife Res Ctr
来源：
ANALYTICAL BIOCHEMISTRY | 1995年 / 226卷 / 02期
关键词：
D O I：
10.1006/abio.1995.1237
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An efficient method for measuring polychlorinated biphenyl-mediated induction of cytochrome P4501A, using the ethoxyresorufin-O-deethylase (EROD) assay, and total porphyrins in chicken embryo hepatocyte cultures is described. Hepatocytes were cultured in 48-well plates, assays were carried out within the wells, and concentrations of the product of the EROD reaction (resorufin), porphyrins, and total proteins were measured in the same wells with a fluorescence plate reader. The method differs from previous methods developed in this laboratory in that three fluorophors were measured in the same well rather than two. (C) 1995 Academic Press, Inc.
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页码：362 / 370
页数：9
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