CLONING AND SEQUENCING OF AN OVINE GRANULOCYTE-COLONY-STIMULATING FACTOR CDNA

被引：5

作者：

OBRIEN, PM ^{[1
]}

SEOW, HF ^{[1
]}

ROTHEL, JS ^{[1
]}

WOOD, PR ^{[1
]}

机构：

[1] CSIRO,DIV ANIM HLTH,ANIM HLTH RES LAB,PARKVILLE,VIC 3052,AUSTRALIA

来源：

DNA SEQUENCE | 1994年 / 4卷 / 05期

关键词：

NEUTROPHIL; NUCLEOTIDE SEQUENCE; OVINE G-CSF; PCR;

D O I：

10.3109/10425179409020862

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cDNA encoding an ovine (ov) granulocyte colony-stimulating factor (C-CSF) was cloned by polymerase chain reaction, using primers based on the 5' and 3' nucleotide (nt) sequence of the bovine (bov) G-CSF cDNA. RNA was isolated from IFN-gamma and LPS-stimulated alveolar macrophages. The isolated oVG-CSF cDNA is 522 bp in length and does not include coding sequence for a secretory signal peptide. The nt sequence is 95% identical to the bovG-CSF nt sequence, and includes conservation of 5 cysteine residues. The deduced amino acid (aa) sequence of the mature ovG-CSF protein shares 96%, 82% and 68% homology with the bov, human and murine mature C-CSF proteins, respectively. The estimated molecular weight of the 174 aa C-CSF protein is 18.8 kilodaltons.

引用

页码：339 / 342

页数：4

共 26 条

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