EFFECT OF ASCORBATE ON LUTEINIZING-HORMONE STIMULATED PROGESTERONE BIOSYNTHESIS IN CHICKEN GRANULOSA-CELLS INVITRO

被引：11
|
作者：
BYRD, JA
PARDUE, SL
HARGIS, BM
机构：
[1] TEXAS A&M UNIV SYST,TEXAS AGR EXPT STN,DEPT POULTRY SCI,COLL STN,TX 77843
[2] TEXAS A&M UNIV SYST,TEXAS AGR EXPT STN,DEPT VET PATHOBIOL,COLL STN,TX 77843
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY | 1993年 / 104卷 / 02期
关键词：
D O I：
10.1016/0300-9629(93)90317-W
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. Utilizing hen granulosa cells from the two largest (F1 and F2) follicles, we have examined the effect of sodium ascorbate (AS) on luteinizing hormone (LH) stimulated progesterone (PR) biosynthesis. 2. In experiment 1, in vitro AS levels as 0.0, 0.2, 0.4 or 0.8 mM were permutated by LH concentrations of 0.0, 1, 10 or 100 ng/ml. 3. Combination of 0.4 or 0.8 mM AS with physiological LH concentrations significantly (P < 0.05) enhanced (up to 28%) PR production. 4. In experiment 2, levels of AS at 0.3 and 0.6 mM significantly increased (up to 29%) LH induced PR synthesis. 5. These findings suggest that AS may be associated with chicken steroidogenesis as has been indicated for mammalian species.
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页码：279 / 281
页数：3
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