AFFINITY PURIFICATION OF HISTIDINE-TAGGED PROTEINS TRANSIENTLY PRODUCED IN HELA-CELLS

被引：37

作者：

JANKNECHT, R ^{[1
]}

NORDHEIM, A ^{[1
]}

机构：

[1] HANNOVER MED SCH,INST MOLEK BIOL,KONSTANTY GUTSCHOW STR 8,W-3000 HANNOVER 61,GERMANY

来源：

GENE | 1992年 / 121卷 / 02期

关键词：

TRANSFECTION; EUKARYOTIC EXPRESSION VECTOR; METAL CHELATE AFFINITY CHROMATOGRAPHY; HUMAN ELK-1 PROTEIN; SERUM-RESPONSE FACTOR; DNA-PROTEIN INTERACTION;

D O I：

10.1016/0378-1119(92)90137-E

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In order to produce eukaryotic proteins in a functional state, it is often necessary to use eukaryotic instead of prokaryotic expression systems. We have designed vectors which can be employed to express either N- or C-terminally histidine-tagged proteins in transiently transfected eukaryotic cells. The histidine tag allows the rapid enrichment of these proteins by metal chelate affinity chromatography in a native and functional state. Yields of up to 5 mug protein/5 X 10(7) cells were achieved.

引用

页码：321 / 324

页数：4

共 12 条

[1]

HARLOW E, 1988, ANTIBODIES LABORATOR

[2] ETS-RELATED PROTEIN ELK-1 IS HOMOLOGOUS TO THE C-FOS REGULATORY FACTOR P62TCF [J].

HIPSKIND, RA ;

RAO, VN ;

MUELLER, CGF ;

REDDY, ESP ;

NORDHEIM, A .

NATURE, 1991, 354 (6354) :531-534

[3] GENETIC APPROACH TO FACILITATE PURIFICATION OF RECOMBINANT PROTEINS WITH A NOVEL METAL CHELATE ADSORBENT [J].

HOCHULI, E ;

BANNWARTH, W ;

DOBELI, H ;

GENTZ, R ;

STUBER, D .

BIO-TECHNOLOGY, 1988, 6 (11) :1321-1325

[4] NEW METAL CHELATE ADSORBENT SELECTIVE FOR PROTEINS AND PEPTIDES CONTAINING NEIGHBORING HISTIDINE-RESIDUES [J].

HOCHULI, E ;

DOBELI, H ;

SCHACHER, A .

JOURNAL OF CHROMATOGRAPHY, 1987, 411 :177-184

[5] RAPID AND EFFICIENT PURIFICATION OF NATIVE HISTIDINE-TAGGED PROTEIN EXPRESSED BY RECOMBINANT VACCINIA VIRUS [J].

JANKNECHT, R ;

DEMARTYNOFF, G ;

LOU, J ;

HIPSKIND, RA ;

NORDHEIM, A ;

STUNNENBERG, HG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (20) :8972-8976

[6] EUKARYOTIC EXPRESSION VECTORS FOR THE ANALYSIS OF MUTANT PROTEINS [J].

MATTHIAS, P ;

MULLER, MM ;

SCHREIBER, E ;

RUSCONI, S ;

SCHAFFNER, W .

NUCLEIC ACIDS RESEARCH, 1989, 17 (15) :6418-6418

[7] ISOLATION AND PROPERTIES OF CDNA CLONES ENCODING SRF, A TRANSCRIPTION FACTOR THAT BINDS TO THE C-FOS SERUM RESPONSE ELEMENT [J].

NORMAN, C ;

RUNSWICK, M ;

POLLOCK, R ;

TREISMAN, R .

CELL, 1988, 55 (06) :989-1003

[8]

RAO VN, 1992, ONCOGENE, V7, P65

[9] ELK, TISSUE-SPECIFIC ETS-RELATED GENES ON CHROMOSOME-X AND CHROMOSOME-14 NEAR TRANSLOCATION BREAKPOINTS [J].

RAO, VN ;

HUEBNER, K ;

ISOBE, M ;

ARRUSHDI, A ;

CROCE, CM ;

REDDY, ESP .

SCIENCE, 1989, 244 (4900) :66-70

[10] MULTIPLE BASAL PROMOTER ELEMENTS DETERMINE THE LEVEL OF HUMAN C-FOS TRANSCRIPTION [J].

RUNKEL, L ;

SHAW, PE ;

HERRERA, RE ;

HIPSKIND, RA ;

NORDHEIM, A .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (03) :1270-1280

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