CRYSTAL-STRUCTURE OF AN N-TERMINAL FRAGMENT OF THE DNA GYRASE B-PROTEIN

被引：502

作者：

WIGLEY, DB ^{[1
]}

DAVIES, GJ ^{[1
]}

DODSON, EJ ^{[1
]}

MAXWELL, A ^{[1
]}

DODSON, G ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT BIOCHEM,LEICESTER LE1 7RH,ENGLAND

来源：

NATURE | 1991年 / 351卷 / 6328期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/351624a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The crystal structure of an N-terminal fragment of the Escherichia coli DNA gyrase B protein, complexed with a nonhydrolysable ATP analogue, has been solved at 2.5 angstrom resolution. It consists of two domains, both containing novel protein folds. The protein fragment forms a dimer, whose N-terminal domains are responsible for ATP binding and hydrolysis. The C-terminal domains form the sides of a 20 angstrom hole through the protein dimer which may play a role in DNA strand passage during the supercoiling reaction.

引用

收藏

页码：624 / 629

页数：6

相关论文

共 26 条

[1] DNA-SEQUENCE OF THE ESCHERICHIA-COLI GYRB GENE - APPLICATION OF A NEW SEQUENCING STRATEGY [J].

ADACHI, T ;

MIZUUCHI, M ;

ROBINSON, EA ;

APPELLA, E ;

ODEA, MH ;

GELLERT, M ;

MIZUUCHI, K .

NUCLEIC ACIDS RESEARCH, 1987, 15 (02) :771-784

[2] TOPOISOMERASE FROM ESCHERICHIA-COLI RELATED TO DNA GYRASE [J].

BROWN, PO ;

PEEBLES, CL ;

COZZARELLI, NR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (12) :6110-6114

[3] CRYSTALLOGRAPHIC REFINEMENT BY SIMULATED ANNEALING APPLICATION TO A 2.8-A RESOLUTION STRUCTURE OF ASPARTATE-AMINOTRANSFERASE [J].

BRUNGER, AT .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (03) :803-816

[4] DNA GYRASE - PURIFICATION AND CATALYTIC PROPERTIES OF A FRAGMENT OF GYRASE-B PROTEIN [J].

GELLERT, M ;

FISHER, LM ;

ODEA, MH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (12) :6289-6293

[5] DNA TOPOISOMERASES [J].

GELLERT, M .

ANNUAL REVIEW OF BIOCHEMISTRY, 1981, 50 :879-910

[6]

Hendrickson WA, 1981, BIOMOLECULAR STRUCTU, V1, P43

[7] PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF THE ATP-HYDROLYZING DOMAIN OF THE ESCHERICHIA-COLI DNA GYRASE B-PROTEIN [J].

JACKSON, AP ;

MAXWELL, A ;

WIGLEY, DB .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 217 (01) :15-17

[8] DNA GYRASE AND ITS COMPLEXES WITH DNA - DIRECT OBSERVATION BY ELECTRON-MICROSCOPY [J].

KIRCHHAUSEN, T ;

WANG, JC ;

HARRISON, SC .

CELL, 1985, 41 (03) :933-943

[9] NEUTRON AND LIGHT-SCATTERING-STUDIES OF DNA GYRASE AND ITS COMPLEX WITH DNA [J].

KRUEGER, S ;

ZACCAI, G ;

WLODAWER, A ;

LANGOWSKI, J ;

ODEA, M ;

MAXWELL, A ;

GELLERT, M .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 211 (01) :211-220

[10] MECHANISTIC ASPECTS OF DNA TOPOISOMERASES [J].

MAXWELL, A ;

GELLERT, M .

ADVANCES IN PROTEIN CHEMISTRY, 1986, 38 :69-107