MODIFICATION OF BRASSICA SEED OIL BY ANTISENSE EXPRESSION OF A STEAROYL-ACYL CARRIER PROTEIN DESATURASE GENE

被引：279

作者：

KNUTZON, DS ^{[1
]}

THOMPSON, GA ^{[1
]}

RADKE, SE ^{[1
]}

JOHNSON, WB ^{[1
]}

KNAUF, VC ^{[1
]}

KRIDL, JC ^{[1
]}

机构：

[1] CALGENE INC, 1920 5TH ST, DAVIS, CA 95616 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 07期

关键词：

FATTY ACID BIOSYNTHESIS; LIPID METABOLISM; RAPESEED; SATURATED FATTY ACIDS;

D O I：

10.1073/pnas.89.7.2624

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions.

引用

页码：2624 / 2628

页数：5

共 50 条

[21] NUCLEOTIDE-SEQUENCE AND TEMPORAL REGULATION OF A SEED-SPECIFIC BRASSICA-NAPUS CDNA-ENCODING A STEAROYL-ACYL CARRIER PROTEIN (ACP) DESATURASE
SLOCOMBE, SP
CUMMINS, I
JARVIS, RP
MURPHY, DJ
PLANT MOLECULAR BIOLOGY, 1992, 20 (01) : 151 - 155
[22] Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis
Liu, Jin
Sun, Zheng
Zhong, Yujuan
Huang, Junchao
Hu, Qiang
Chen, Feng
PLANTA, 2012, 236 (06) : 1665 - 1676
[23] Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis
Jin Liu
Zheng Sun
Yujuan Zhong
Junchao Huang
Qiang Hu
Feng Chen
Planta, 2012, 236 : 1665 - 1676
[24] Characterization of a stearoyl-acyl carrier protein desaturase gene from potential biofuel plant, Pongamia pinnata L.
Ramesh, Aadi Moo Lam
Kesari, Vigya
Rangan, Latha
GENE, 2014, 542 (02) : 113 - 121
[25] Modification of the fatty acid composition in Arabidopsis and maize seeds using a stearoyl-acyl carrier protein desaturase-1 (ZmSAD1) gene
Hewei Du
Min Huang
Jieyun Hu
Jiansheng Li
BMC Plant Biology, 16
[26] Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley
Ding, Mingyu
Zhou, Danni
Ye, Yichen
Wen, Shuting
Zhang, Xian
Tian, Quanxiang
Zhang, Xiaoqin
Mou, Wangshu
Dang, Cong
Fang, Yunxia
Xue, Dawei
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (01)
[27] Modification of the fatty acid composition in Arabidopsis and maize seeds using a stearoyl-acyl carrier protein desaturase-1 (ZmSAD1) gene
Du, Hewei
Huang, Min
Hu, Jieyun
Li, Jiansheng
BMC PLANT BIOLOGY, 2016, 16
[28] NUCLEOTIDE-SEQUENCE OF A STEAROYL-ACYL CARRIER PROTEIN DESATURASE CDNA FROM DEVELOPING SEEDS OF RICE
AKAGI, H
BABA, T
SHIMADA, H
FUJIMURA, T
PLANT PHYSIOLOGY, 1995, 108 (02) : 845 - 846
[29] Constitutive BiP protein accumulation in Arabidopsis mutants defective in a gene encoding chloroplast-resident stearoyl-acyl carrier protein desaturase
Iwata, Yuji
Iida, Tsukasa
Matsunami, Toshihiro
Yamada, Yu
Mishiba, Kei-ichiro
Ogawa, Takumi
Kurata, Tetsuya
Koizumi, Nozomu
GENES TO CELLS, 2018, 23 (06) : 456 - 465
[30] A stearoyl-acyl carrier protein desaturase, NbSACPD-C, is critical for ovule development in Nicotiana benthamiana
Zhang, Jiantao (jtzhang@vt.edu), 1600, Blackwell Publishing Ltd (80):

← 1 2 3 4 5 →