MODIFICATION OF BRASSICA SEED OIL BY ANTISENSE EXPRESSION OF A STEAROYL-ACYL CARRIER PROTEIN DESATURASE GENE

被引：282

作者：

KNUTZON, DS ^{[1
]}

THOMPSON, GA ^{[1
]}

RADKE, SE ^{[1
]}

JOHNSON, WB ^{[1
]}

KNAUF, VC ^{[1
]}

KRIDL, JC ^{[1
]}

机构：

[1] CALGENE INC, 1920 5TH ST, DAVIS, CA 95616 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 07期

关键词：

FATTY ACID BIOSYNTHESIS; LIPID METABOLISM; RAPESEED; SATURATED FATTY ACIDS;

D O I：

10.1073/pnas.89.7.2624

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions.

引用

页码：2624 / 2628

页数：5

共 31 条

[1] PROPERTIES OF THE GLYCEROL ACYLATING ENZYMES IN MICROSOMAL PREPARATIONS FROM THE DEVELOPING SEEDS OF SAFFLOWER (CARTHAMUS-TINCTORIUS) AND TURNIP RAPE (BRASSICA-CAMPESTRIS) AND THEIR ABILITY TO ASSEMBLE COCOA-BUTTER TYPE FATS [J].