MODIFICATION OF BRASSICA SEED OIL BY ANTISENSE EXPRESSION OF A STEAROYL-ACYL CARRIER PROTEIN DESATURASE GENE

被引：279
|
作者：
KNUTZON, DS [1 ]
THOMPSON, GA [1 ]
RADKE, SE [1 ]
JOHNSON, WB [1 ]
KNAUF, VC [1 ]
KRIDL, JC [1 ]
机构：
[1] CALGENE INC, 1920 5TH ST, DAVIS, CA 95616 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 07期
关键词：
FATTY ACID BIOSYNTHESIS; LIPID METABOLISM; RAPESEED; SATURATED FATTY ACIDS;
D O I：
10.1073/pnas.89.7.2624
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions.
引用
收藏
页码：2624 / 2628
页数：5
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