COUNTERCURRENT CHROMATOGRAPHIC ISOLATION OF HIGH-DENSITY-LIPOPROTEIN AND LOW-DENSITY-LIPOPROTEIN FRACTIONS FROM HUMAN SERUM

被引：0

作者：

SHIBUSAWA, Y ^{[1
]}

CHIBA, T ^{[1
]}

MATSUMOTO, U ^{[1
]}

ITO, Y ^{[1
]}

机构：

[1] NHLBI, BIOPHYS CHEM LAB, BETHESDA, MD 20892 USA

来源：

MODERN COUNTERCURRENT CHROMATOGRAPHY | 1995年 / 593卷

关键词：

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

High- and low-density lipoproteins (HDLs, LDLs) were fractionated by countercurrent chromatography using the type-XL cross-axis coil planet centrifuge using two-phase aqueous polymer systems. The best separations were achieved with a polymer phase system composed of 16%(w/w) polyethylene glycol 1000 and 12.5%(w/w) dibasic potassium phosphate at pH 9.4 by eluting the lower phase at 2.0 ml/min. HDL-LDL fractions containing 1.26, 1.27 and 0.42 mg/ml of phospholipids, cholesterols and triglycerides, respectively, were collected from 4 ml of human serum within 3 h. The presence of HDLs and LDLs were confirmed by gel electrophoresis with Sudan Black B staining. The HDL-LDL fractions were found to be free of serum proteins in SDS polyacrylamide slab gel electrophoresis.

引用

页码：119 / 128

页数：10

共 15 条

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