RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR TOTAL HOMOCYSTEINE LEVELS IN HUMAN SERUM

被引：631
作者：
UBBINK, JB
VERMAAK, WJH
BISSBORT, S
机构：
[1] Department of Chemical Pathology, Faculty of Medicine, University of Pretoria, Pretoria, 0001
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期
关键词：
D O I：
10.1016/0378-4347(91)80407-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A rapid, isocratic high-performance liquid chromatographic (HPLC) method is described for the determination of total homocysteine levels in human serum. Prior to reversed-phase HPLC analysis, the serum thiols were derivatized with SBD-F (ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate), a thiol-specific fluorogenic probe which is commercially available. Retention of SBD-homocysteine was sensitive to pH, and a mobile phase pH of 2.1 ensured baseline separation of serum thiols within 6 min. The method is simple, sensitive, reproducible (between-run coefficient of variation of 6.6%) and very suitable for routine determination of serum homocysteine levels in a clinical pathology laboratory.
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收藏
页码：441 / 446
页数：6
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