A CHEMICALLY-DEFINED MEDIUM FOR ORGANOTYPIC SLICE CULTURES

被引：47

作者：

ANNIS, CM

EDMOND, J

ROBERTSON, RT

机构：

[1] UNIV CALIF LOS ANGELES,DEPT BIOL CHEM,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,MENTAL RETARDAT RES CTR,LOS ANGELES,CA 90024

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1990年 / 32卷 / 01期

关键词：

Cerebral cortex; Defined medium; Development; Organotypic tissue culture;

D O I：

10.1016/0165-0270(90)90072-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Organotypic slice cultures provide an excellent system for the analysis of axonal connectivity and development. However, the study of the molecular mechanisms of this development necessitates the use of a chemically defined culture medium. We report here the development of a medium, EOL1 defined medium, designed specifically for this purpose. Cultures of both cerebral cortex and basal forebrain demonstrate that this defined medium allows a high degree of cytoarchitectural maintenance while promoting neural metabolism and process outgrowth. © 1990.

引用

页码：63 / 70

页数：8

共 23 条

[1]

BORNSTEIN MB, 1958, LAB INVEST, V7, P134

[2]

BRUNNER G, 1982, DEV BRAIN RES, V2, P563

[3]

Crain S M, 1966, Int Rev Neurobiol, V9, P1, DOI 10.1016/S0074-7742(08)60135-X

[4] CYTOARCHITECTURE IN CULTURED RAT NEOCORTEX EXPLANTS [J].