A total of 85 blood samples, obtained from Yugoslav trotter horses were analysed for serum protein polymorphism at the following loci: albumin (Al), protease inhibitor (Pi), transferrin (Tf), esterase (Es) and Xk protein by standard starch gel electrophoretic procedures. From the results obtained the homogeneity index and parentage exclusion probability were calculated. The characteristic gene frequencies of the investigated Al, Pi, Ti, Es and Xk protein systems were established as: AIA and AIB (0.424 and 0.576); PiF, PiL, PiG, Pil, PiV and PiS (0.135, 0.318, 0.123, 0.100, 0.259 and 0.576); TiD, TiF, TiH and TiO (0.359, 0.529, 0.036 and 0.076), EsF, EsI and EsS (0.265, 0.570 and 0.165); and XkK and XkS (0.912 and 0.088), respectively. The Homogeneity index of the tested population was 0.0049, 0.5755, 0.2209, 0.1336 and 0.6790 for the AL, Pi, Tf, Es and Xk, loci, respectively. The joint paternity exclusion probability was 83.40% for the population of Yugoslav trotters.