INTERACTION OF DNA WITH CATIONIC LIPOSOMES - ABILITY OF TRANSFECTING LENTIL PROTOPLASTS

被引：25

作者：

MACCARRONE, M

DINI, L

DIMARZIO, L

DIGIULIO, A

ROSSI, A

MOSSA, G

FINAZZIAGRO, A

机构：

[1] UNIV ROMA TOR VERGATA, DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM, VIA O RAIMONDO, I-00173 ROME, ITALY

[2] CNR, IST MED SPERIMENTALE, ROME, ITALY

[3] UNIV LAQUILA, DIPARTIMENTO SCI & TECNOL BIOMED & BIOMETRIA, I-67100 LAQUILA, ITALY

[4] UNIV ROMA TOR VERGATA, DIPARTIMENTO BIOL, ROME, ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 186卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)81564-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The vesicles made of dipalmitoylphosphatidylcholine and stearylamine (9:1) were multilamellar and rather homogeneous in shape as seen by transmission electron microscopy. Upon addition of circular DNA plasmids of different lengths to the liposomes, the formation of vesicle clusters around the DNA filament was observed, with dimensions dictated by the ratio DNA/lipid. These liposomes were able to transfect lentil (Lens culinaris) protoplasts inside the cells two different reporter genes, chloramphenicol-acetyltransferase and β-glucuronidase. The activity of these two enzymes could be found in the cell lysates after 24 h from the incubation of protoplasts with the lipid-DNA complexes. © 1992 Academic Press, Inc.

引用

页码：1417 / 1422

页数：6

共 33 条

[1] LIPOSOMES BEARING A QUATERNARY AMMONIUM DETERGENT AS AN EFFICIENT VEHICLE FOR FUNCTIONAL TRANSFER OF TMV-RNA INTO PLANT-PROTOPLASTS [J].