INTRODUCTION OF THE ESCHERICHIA-COLI GDHA GENE INTO RHIZOBIUM-PHASEOLI - EFFECT ON NITROGEN-FIXATION

被引：18

作者：

BRAVO, A

BECERRIL, B

MORA, J

机构：

[1] UNIV NACL AUTONOMA MEXICO, CTR INVEST FIJAC NITROGENO, APARTADO POSTAL 565-A, CUERNAVACA, MORELOS, MEXICO

[2] UNIV NACL AUTONOMA MEXICO, CTR INGN GENET & BIOTECHNOL, CUERNAVACA, MORELOS, MEXICO

来源：

JOURNAL OF BACTERIOLOGY | 1988年 / 170卷 / 02期

关键词：

D O I：

10.1128/jb.170.2.985-988.1988

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rhizobium phaseoli lacks glutamate dehydrogenase (GDH) and assimilates ammonium by the glutamine synthetase-glutamate synthase pathway. A strain of R. phaseoli harboring the Escherichia coli GDH structural gene (gdhA) was constructed. GDH activity was expressed in R. phaseoli in the free-living state and in symbiosis. Nodules with bacteroids that expressed GDH activity had severe impairment of nitrogen fixation. Also, R. phaseoli cells that lost GDH activity and assimilated ammonium by the glutamine synthetase-glutamate synthase pathway preferentially nodulated Phaseolus vulgaris.

引用

页码：985 / 988

页数：4

共 27 条

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