INTERACTION OF TYROSINE-BASED SORTING SIGNALS WITH CLATHRIN-ASSOCIATED PROTEINS

被引：836

作者：

OHNO, H

STEWART, J

FOURNIER, MC

BOSSHART, H

RHEE, I

MIYATAKE, S

SAITO, T

GALLUSSER, A

KIRCHHAUSEN, T

BONIFACINO, JS

机构：

[1] NICHHD, CELL BIOL & METAB BRANCH, BETHESDA, MD 20892 USA

[2] HARVARD UNIV, SCH MED, DEPT CELL BIOL, BOSTON, MA 02115 USA

[3] HARVARD UNIV, SCH MED, CTR BLOOD RES, BOSTON, MA 02115 USA

[4] CHIBA UNIV, SCH MED, CTR BIOMED SCI, DIV MOLEC GENET, CHIBA 260, JAPAN

来源：

SCIENCE | 1995年 / 269卷 / 5232期

关键词：

D O I：

10.1126/science.7569928

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tyrosine-based signals within the cytoplasmic domain of integral membrane proteins mediate clathrin-dependent protein sorting in the endocytic and secretory pathways. A yeast two-hybrid system was used to identify proteins that bind to tyrosine-based signals. The medium chains (mu(1) and mu(2)) of two clathrin-associated protein complexes (AP-1 and AP-2, respectively) specifically interacted with tyrosine-based signals of several integral membrane proteins. The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery.

引用

页码：1872 / 1875

页数：4

共 35 条

[1] INVITRO BINDING OF THE ASIALOGLYCOPROTEIN RECEPTOR TO THE BETA ADAPTIN OF PLASMA-MEMBRANE COATED VESICLES [J].