4-O-BETA-D-GALACTOPYRANOSYL-D-XYLOSE - A NEW SYNTHESIS AND APPLICATION TO THE EVALUATION OF INTESTINAL LACTASE

被引：22
|
作者：
RIVERASAGREDO, A
FERNANDEZMAYORALAS, A
JIMENEZBARBERO, J
MARTINLOMAS, M
VILLANUEVA, D
ARAGON, JJ
机构：
[1] CSIC,INST QUIM ORGAN,CARBOHIDRATOS GRP,JUAN DE LA CIERVA 3,E-28006 MADRID,SPAIN
[2] UNIV AUTONOMA MADRID,FAC MED,DEPT BIOQUIM,E-28029 MADRID,SPAIN
来源：
CARBOHYDRATE RESEARCH | 1992年 / 228卷 / 01期
关键词：
D O I：
10.1016/S0008-6215(00)90554-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
4-O-beta-D-Galactopyranosyl-D-xylose (2) was prepared from benzyl 2,3-O-isopropylidene-beta-D-xylopyranoside by glycosylation with 2,3,4,6-tetra-O-benzoyl-alpha-D-galactopyranosyl bromide and subsequent deprotection. Compound 2 was hydrolyzed in vitro by intestinal lactase; the V(max) was 25% of that with lactose and the K(m) was 370mM (cf. 27mM for lactose). Oral administration of 2 to suckling rats led to urinary excretion of D-xylose which could be estimated colorimetrically.
引用
收藏
页码：129 / 135
页数：7
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