CHARACTERIZATION OF THE 5' FLANKING REGION OF THE HUMAN FOLLICLE-STIMULATING-HORMONE RECEPTOR GENE

被引：55
作者：
GROMOLL, J [1 ]
DANKBAR, B [1 ]
GUDERMANN, T [1 ]
机构：
[1] UNIV MUNSTER,INST REPROD MED,D-48149 MUNSTER,GERMANY
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1994年 / 102卷 / 1-2期
关键词：
HUMAN FOLLICLE-STIMULATING HORMONE RECEPTOR GENE; 5' FLANKING REGION; PROMOTER; CAT ACTIVITY; TRANSCRIPTION START SITE;
D O I：
10.1016/0303-7207(94)90102-3
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A genomic clone containing 2.3 kilobases (kb) of the 5' flanking region of the human follicle-stimulating hormone receptor (FSHR) plus the translated region of exon 1 and subsequent sequences of intron A has been isolated and characterized. This portion of the 5' flanking region has neither a TATA nor a CCAAT box and shows features of promoters seen in ''housekeeping'' genes. Using RNAse protection multiple transcriptional start sites could be identified, the major ones clustered between -114 and -79 bp. Chimeras containing 1486 bp of the 5' flanking region, or deletions thereof, expressed significant chloramphenicol acetyltransferase (CAT) activity when transiently transfected into Chinese hamster ovary (CHO), primary rat Sertoli and human granulosa-lutein cells. Deletion analyses indicated that a proximal promoter can be allocated to the region from -225 to -1 bp.
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页码：93 / 102
页数：10
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