INHIBITION OF HEAT INACTIVATION OF REVERSE-TRANSCRIPTASE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 BY SEROPOSITIVE SERA

被引：5
作者：
NAGUMO, T [1 ]
TAKEUCHI, Y [1 ]
HOSHINO, H [1 ]
机构：
[1] GUNMA UNIV, SCH MED, DEPT HYG & VIROL, 3-39-22 SHOWA MACHI, MAEBASHI, GUNMA 371, JAPAN
来源：
JAPANESE JOURNAL OF CANCER RESEARCH | 1992年 / 83卷 / 06期
关键词：
HIV-1; REVERSE TRANSCRIPTASE; AIDS; ANTIBODY;
D O I：
10.1111/j.1349-7006.1992.tb00131.x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The reverse transcriptase (RT) activity of a lysate of human immunodeficiency virus type 1 (HIV) was almost completely inactivated by incubation at 56-degrees-C for 20-30 min. The heat-inactivation of RT in the virus lysate or purified RT was partially inhibited in the presence of some human sera or plasma containing antibodies against HIV. The IgG fraction purified from the seropositive sera was responsible for stabilization of RT upon heat inactivation. This is a new assay system for detection of antibodies against RT.
引用
收藏
页码：594 / 600
页数：7
相关论文
共 24 条
  • [1] ALLAN JS, 1987, BLOOD, V69, P331
  • [2] MAJOR GLYCOPROTEIN ANTIGENS THAT INDUCE ANTIBODIES IN AIDS PATIENTS ARE ENCODED BY HTLV-III
    ALLAN, JS
    COLIGAN, JE
    BARIN, F
    MCLANE, MF
    SODROSKI, JG
    ROSEN, CA
    HASELTINE, WA
    LEE, TH
    ESSEX, M
    [J]. SCIENCE, 1985, 228 (4703) : 1091 - 1094
  • [3] VIRUS ENVELOPE PROTEIN OF HTLV-III REPRESENTS MAJOR TARGET ANTIGEN FOR ANTIBODIES IN AIDS PATIENTS
    BARIN, F
    MCLANE, MF
    ALLAN, JS
    LEE, TH
    GROOPMAN, JE
    ESSEX, M
    [J]. SCIENCE, 1985, 228 (4703) : 1094 - 1096
  • [4] ISOLATION OF A T-LYMPHOTROPIC RETROVIRUS FROM A PATIENT AT RISK FOR ACQUIRED IMMUNE-DEFICIENCY SYNDROME (AIDS)
    BARRESINOUSSI, F
    CHERMANN, JC
    REY, F
    NUGEYRE, MT
    CHAMARET, S
    GRUEST, J
    DAUGUET, C
    AXLERBLIN, C
    VEZINETBRUN, F
    ROUZIOUX, C
    ROZENBAUM, W
    MONTAGNIER, L
    [J]. SCIENCE, 1983, 220 (4599) : 868 - 871
  • [5] BIOCHEMICAL HETEROGENEITY OF REVERSE-TRANSCRIPTASE PURIFIED FROM THE AIDS VIRUS, HTLV-III
    CHANDRA, A
    GERBER, T
    CHANDRA, P
    [J]. FEBS LETTERS, 1986, 197 (1-2) : 84 - 88
  • [6] FREQUENT DETECTION AND ISOLATION OF CYTOPATHIC RETROVIRUSES (HTLV-III) FROM PATIENTS WITH AIDS AND AT RISK FOR AIDS
    GALLO, RC
    SALAHUDDIN, SZ
    POPOVIC, M
    SHEARER, GM
    KAPLAN, M
    HAYNES, BF
    PALKER, TJ
    REDFIELD, R
    OLESKE, J
    SAFAI, B
    WHITE, G
    FOSTER, P
    MARKHAM, PD
    [J]. SCIENCE, 1984, 224 (4648) : 500 - 503
  • [7] HUMAN T-CELL LEUKEMIA-VIRUS IS NOT LYSED BY HUMAN-SERUM
    HOSHINO, H
    TANAKA, H
    MIWA, M
    OKADA, H
    [J]. NATURE, 1984, 310 (5975) : 324 - 325
  • [8] ETIOLOGY OF AIDS - ANTIBODIES TO HUMAN T-CELL LEUKEMIA-VIRUS (TYPE-III) IN HEMOPHILIACS
    KITCHEN, LW
    BARIN, F
    SULLIVAN, JL
    MCLANE, MF
    BRETTLER, DB
    LEVINE, PH
    ESSEX, M
    [J]. NATURE, 1984, 312 (5992) : 367 - 369
  • [9] PERSISTENT HIV ANTIGENEMIA AND DECLINE OF HIV CORE ANTIBODIES ASSOCIATED WITH TRANSITION TO AIDS
    LANGE, JMA
    PAUL, DA
    HUISMAN, HG
    DEWOLF, F
    VANDENBERG, H
    COUTINHO, RA
    DANNER, SA
    VANDERNOORDAA, J
    GOUDSMIT, J
    [J]. BRITISH MEDICAL JOURNAL, 1986, 293 (6560) : 1459 - 1462
  • [10] CHARACTERIZATION AND CLINICAL-ASSOCIATION OF ANTIBODY INHIBITORY TO HIV REVERSE-TRANSCRIPTASE ACTIVITY
    LAURENCE, J
    SAUNDERS, A
    KULKOSKY, J
    [J]. SCIENCE, 1987, 235 (4795) : 1501 - 1504
123