CDNA CLONING AND MEMBRANE TOPOLOGY OF THE RABBIT GASTRIC H+/K+-ATPASE ALPHA-SUBUNIT

被引：61

作者：

BAMBERG, K ^{[1
]}

MERCIER, F ^{[1
]}

REUBEN, MA ^{[1
]}

KOBAYASHI, Y ^{[1
]}

MUNSON, KB ^{[1
]}

SACHS, G ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT PHYSIOL,LOS ANGELES,CA 90024

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 01期

关键词：

H+/K+-ATPASE; CDNA CLONING; TOPOLOGY; ANTIBODY;

D O I：

10.1016/0167-4781(92)90100-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced a cDNA for the rabbit gastric proton-potassium pump (H+/K+-ATPase) alpha-subunit. The deduced peptide contains 1035 amino acids (M(r) 114201) and shows 97% sequence identity with the respective rat and hog proteins. A monoclonal antibody 146-14 has been shown previously to react with thc extracytoplasmic side of the catalytic H+/K+-ATPase subunit and here we show that the epitope is in the region between amino acids 855 and 902 (the numbering of the H+/K+-ATPase catalytic subunit throughout the paper refers to thc rabbit sequence). The localization of this epitope in conjunction with previously observed trypsin cleavage sites in the C-terminal one third of the enzyme and the hydrophobicity plot of the deduced peptide sequence are evidence for a structural model for the alpha-subunit of the H+/K+-ATPase which contains at least ten membrane spanning segments, similar to that deduced for the Ca2+-ATPase of sarcoplasmic reticulum.

引用

页码：69 / 77

页数：9

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