SIMULTANEOUS DETERMINATION OF A NOVEL ANGIOTENSIN-II RECEPTOR BLOCKING-AGENT, LOSARTAN, AND ITS METABOLITE IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：97

作者：

FURTEK, CI ^{[1
]}

LO, MW ^{[1
]}

机构：

[1] MERCK SHARP & DOHME LTD,DEPT DRUG METAB,W POINT,PA 19486

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 573卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80132-A

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and selective high-performance liquid chromatographic method for the simultaneous determination of a new angiotensin II receptor blocking agent, losartan (DuP 753, MK-954, I), and its active metabolite, EXP3174 (II), in human plasma or urine is described. The two analytes and internal standard are extracted from plasma and urine at pH 2.5 by liquid-liquid extraction and analyzed on a cyano column with ultraviolet detection at 254 nm. The mobile phase is composed of acetonitrile and phosphate buffer at pH 2.5. The limit of quantification for both compounds in plasma is 5 ng/ml. The limit in urine is 20 and 10 ng/ml for I and II, respectively. The assay described has been successfully applied to samples from pharmacokinetic studies.

引用

页码：295 / 301

页数：7

共 7 条

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[3] FCHRIST DD, 1990, 74TH ANN FASEB M WAS, pA462
[4] NONPEPTIDE ANGIOTENSIN-II RECEPTOR ANTAGONISTS - STUDIES WITH EXP9270 AND DUP753
WONG, PC
PRICE, WA
CHIU, AT
CARINI, DJ
DUNCIA, JV
JOHNSON, AL
WEXLER, RR
TIMMERMANS, BMWM
[J]. HYPERTENSION, 1990, 15 (06) : 823 - 834
[5] WONG PC, 1990, J PHARMACOL EXP THER, V252, P726
[6] WONG PC, 1990, J PHARMACOL EXP THER, V255, P211
[7] WONG PC, 1990, J PHARMACOL EXP THER, V252, P719

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