THE IMPORTANCE OF EARLY FIXATION IN PRESERVATION OF HUMAN COCHLEAR AND VESTIBULAR SENSORY HAIR BUNDLES

被引：9

作者：

COMIS, SD ^{[1
]}

OSBORNE, MP ^{[1
]}

OCONNELL, J ^{[1
]}

JOHNSON, AP ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,SCH MED,DEPT OTOLARYNGOL,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

来源：

ACTA OTO-LARYNGOLOGICA | 1990年 / 109卷 / 5-6期

关键词：

Cross-links; Fixation; Glutaraldehyde; Human cochleascanning electron microscopy;

D O I：

10.3109/00016489009125156

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Human labyrinths were fixed with glutaraldehyde as soon as possible and certainly within 15 min of clinically confirmed death. By following this protocol, we have obtained excellent preservation of human material comparable to the best that we were able to obtain with animal tissues. Structural features that are indicative of good fixation in animal hair bundles, namely well-defined lateral cross-hnks, upward pointing tip links and the 'granular' appearance of the surface membrane of stereocilia are confirmed for human hair cells. Our results show that excellent preservation of human hair bundles is critically dependent upon early fixation. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：361 / 368

页数：8

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