RECOGNITION OF DIFFERENT NUCLEOTIDE-BINDING SITES IN PRIMARY STRUCTURES USING A PROPERTY-PATTERN APPROACH

Consensus sequence patterns for β‐α‐β folds binding FAD, NAD and GTP were constructed on the basis of 11 steric and physicochemical properties. These property patterns permit detection and distinction of the respective nucleotide‐binding sites on the basis of amino acid sequence analysis alone. The SWISS‐PROT database (release 9) was screened with the three calculated patterns, and nucleotide‐binding sites identified are presented. They correspond to existing structure data (if known). For the detected sequence segments we are able to predict the β‐α‐β motif as well as the respective binding sites. For some of the proteins so detected a nucleotide‐binding capacity has not previously been reported. Copyright © 1990, Wiley Blackwell. All rights reserved