ORAL-SUSCEPTIBILITY OF AEDES ALBOPICTUS TO DENGUE TYPE-2 VIRUS - A STUDY OF INFECTION KINETICS, USING THE POLYMERASE CHAIN-REACTION FOR VIRAL DETECTION

被引：1
作者：
TARDIEUX, I
POUPEL, O
RODHAIN, F
LAPCHIN, L
机构：
[1] Unité d'Ecologie des Systèmes Vectoriels, Institut Pasteur, Paris, 75724
[2] I.N.R.A, Laboratoire de Dynamique Et Génétique Des Populations, Antibes, 06600
来源：
MEDICAL AND VETERINARY ENTOMOLOGY | 1992年 / 6卷 / 04期
关键词：
AEDES-ALBOPICTUS; DENGUE SEROTYPE-2; DEN-2; IFA; DNA PROBE; PCR TECHNIQUE; ARBOVIRUS VECTOR; VIRUS KINETICS; VECTOR COMPETENCE;
D O I：
10.1111/j.1365-2915.1992.tb00626.x
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
Female Aedes albopictus mosquitoes, aged 1 week, were infected with DEN-2 dengue virus. The kinetics of infection in mosquito brain and mesenteron were monitored using DNA probes with polymerase chain reaction (PCR) amplification of target DNA sequences coding for DEN-2 virus envelope protein, compared with the standard immunofluorescence assay technique (IFA). Rates of virus detection in the mesenteron of orally infected mosquitoes rose to 38% by day 4 post-inoculation, then declined until day 8, followed by irregular peaks around days 11 - 14 and subsequently. In mosquito head squashes, virus was detected from day 4 onwards, reaching 38% positive by day 18. Salivary glands of all the same females were found to be positive for virus by day 8 onwards. Parenterally infected Ae.albopictus females were all positive for DEN-2 in the brain and salivary glands 8 days post-inoculation. In every case, results obtained with the PCR matched those from the IFA. Our DNA probe with PCR procedure can therefore be utilized as a sensitive and reliable method for studies of DEN-2 vectors.
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页码：311 / 317
页数：7
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