ONSET OF EXPRESSION AND REGIONAL DEPOSITION OF ALPHA-SMOOTH AND SARCOMERIC ACTIN DURING AVIAN HEART DEVELOPMENT

被引:54
|
作者
SUGI, Y
LOUGH, J
机构
[1] Department of Cell Biology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin
关键词
SMOOTH MUSCLE ALPHA-ACTIN; SARCOMERIC ALPHA-ACTIN; EARLY HEART DEVELOPMENT; IMMUNOCYTOCHEMISTRY;
D O I
10.1002/aja.1001930203
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The sequential appearance of mRNAs for smooth, cardiac, and skeletal alpha-actin has been described during development of the chicken heart (Ruzicka, D.L., and R.J. Schwartz 1988 J. Cell Biol., 107.2575-2586). To assess whether this reflects the deposition of corresponding isoproteins, we have immunocytochemically localized smooth and sarcomeric (cardiac and skeletal) alpha-actin in Hamburger-Hamilton (H-H) stage 7-18 embryos using monoclonal antibodies. Within the developing embryo at stage 9-, smooth muscle alpha-actin was exclusively detected in the developing heart, upon fusion of the endocardial tubes; sarcomeric alpha-actin was observed later (stage 9). By the onset of contraction at stage 10+, intense immunostaining of both smooth and sarcomeric isoproteins was observed in the ventricle; at this time smooth muscle alpha-actin was also detected in splanchnic mesoderm of the pre-vitelline area, in a cellular layer adjacent to the only embryonic cells that exhibited factor VIII (von Willebrand factor) antigens. Double immunostaining of the myocardium at stage 11, at which time striations were first detected, revealed the co-existence of smooth and sarcomeric actin in developing sarcomeres. Intense expression of sarcomeric actin continued in the heart after stage 11, whereas smooth muscle alpha-actin was down-regulated in the ventricle and became regionalized to the inflow and outflow tracts. As expected, smooth muscle alpha-actin was detected around intra- and extra-embryonic vascular structures at later developmental stages, while sarcomeric actin was observed in somites.
引用
收藏
页码:116 / 124
页数:9
相关论文
共 50 条
  • [41] Inhibition of alpha-smooth muscle actin expression in an in vitro wound healing model by certain antibiotics
    Yu, PY
    Vlahos, AL
    Dombi, GW
    Ledgerwood, AM
    Lucas, CE
    JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, 1999, 47 (01): : 130 - 135
  • [42] Alpha-smooth muscle actin expression and structure integrity in chondrogenesis of human mesenchymal stem cells
    Hung, SC
    Kuo, PY
    Chang, CF
    Chen, TH
    Ho, LLT
    CELL AND TISSUE RESEARCH, 2006, 324 (03) : 457 - 466
  • [43] ALPHA-SMOOTH MUSCLE ACTIN IS TRANSIENTLY EXPRESSED BY MYOFIBROBLASTS DURING EXPERIMENTAL WOUND-HEALING
    DARBY, I
    SKALLI, O
    GABBIANI, G
    LABORATORY INVESTIGATION, 1990, 63 (01) : 21 - 29
  • [44] The expression of the receptor for hyaluronan mediated motility (RHAMM) and alpha-smooth muscle actin in glomerulonephritis.
    Brophy, PD
    Gough, J
    Liu, B
    Ogborn, MR
    Savani, RC
    Grimm, PC
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 1997, 8 : A2381 - A2381
  • [45] EXPRESSION OF ALPHA-SMOOTH MUSCLE ACTIN IN LIPOCYTES IN HEPATIC-FIBROSIS - AN EVIDENCE FOR MODULATION IN LIPOCYTES
    TANAKA, Y
    NOUCHI, T
    YAMANE, M
    YAMAOKA, K
    IRIE, T
    SATO, C
    MARUMO, F
    HEPATOLOGY, 1990, 12 (04) : 953 - 953
  • [46] CALDESMON, CALPONIN AND ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION IN SUBCULTURED SMOOTH-MUSCLE CELLS FROM HUMAN AIRWAYS
    DURANDARCZYNSKA, W
    MARMY, N
    DURAND, J
    HISTOCHEMISTRY, 1993, 100 (06) : 465 - 471
  • [47] TGF-beta induced expression of alpha-smooth muscle actin is dependent on collagen substrate.
    Narani, N
    Arora, PD
    McCulloch, CAG
    JOURNAL OF DENTAL RESEARCH, 1997, 76 : 2724 - 2724
  • [48] Alpha-Smooth Muscle Actin Expression and Parafoveal Blood Flow Pathways Are Altered in Preclinical Diabetic Retinopathy
    An, Dong
    Chung-Wah-Cheong, Jonathan
    Yu, Dao-Yi
    Balaratnasingam, Chandrakumar
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2022, 63 (05)
  • [49] EXPRESSION OF ALPHA-SMOOTH MUSCLE (A-SM) ACTIN IN THE DEVELOPING KIDNEY VASCULATURE AND IN MODELS OF KIDNEY INJURY
    CAREY, AV
    CAREY, RM
    PUPILLI, C
    CHEVALIER, RL
    GOMEZ, RA
    HYPERTENSION, 1991, 17 (03) : 439 - 439
  • [50] CYTOKINES REGULATE THE EXPRESSION OF CONNEXINS AND ALPHA-SMOOTH MUSCLE CELL ACTIN IN CULTURED HUMAN SUBUROTHELIAL MYOFIBROBLASTS
    Neuhaus, J.
    Heinrich, M.
    Schwalenberg, T.
    Stolzenburg, J. U.
    EUROPEAN UROLOGY SUPPLEMENTS, 2009, 8 (04) : 274 - 274