ONSET OF EXPRESSION AND REGIONAL DEPOSITION OF ALPHA-SMOOTH AND SARCOMERIC ACTIN DURING AVIAN HEART DEVELOPMENT

被引:54
|
作者
SUGI, Y
LOUGH, J
机构
[1] Department of Cell Biology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin
关键词
SMOOTH MUSCLE ALPHA-ACTIN; SARCOMERIC ALPHA-ACTIN; EARLY HEART DEVELOPMENT; IMMUNOCYTOCHEMISTRY;
D O I
10.1002/aja.1001930203
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The sequential appearance of mRNAs for smooth, cardiac, and skeletal alpha-actin has been described during development of the chicken heart (Ruzicka, D.L., and R.J. Schwartz 1988 J. Cell Biol., 107.2575-2586). To assess whether this reflects the deposition of corresponding isoproteins, we have immunocytochemically localized smooth and sarcomeric (cardiac and skeletal) alpha-actin in Hamburger-Hamilton (H-H) stage 7-18 embryos using monoclonal antibodies. Within the developing embryo at stage 9-, smooth muscle alpha-actin was exclusively detected in the developing heart, upon fusion of the endocardial tubes; sarcomeric alpha-actin was observed later (stage 9). By the onset of contraction at stage 10+, intense immunostaining of both smooth and sarcomeric isoproteins was observed in the ventricle; at this time smooth muscle alpha-actin was also detected in splanchnic mesoderm of the pre-vitelline area, in a cellular layer adjacent to the only embryonic cells that exhibited factor VIII (von Willebrand factor) antigens. Double immunostaining of the myocardium at stage 11, at which time striations were first detected, revealed the co-existence of smooth and sarcomeric actin in developing sarcomeres. Intense expression of sarcomeric actin continued in the heart after stage 11, whereas smooth muscle alpha-actin was down-regulated in the ventricle and became regionalized to the inflow and outflow tracts. As expected, smooth muscle alpha-actin was detected around intra- and extra-embryonic vascular structures at later developmental stages, while sarcomeric actin was observed in somites.
引用
收藏
页码:116 / 124
页数:9
相关论文
共 50 条
  • [31] Inhibitory effect of nicotine on collagen and alpha-smooth muscle actin expression in cardiac fibroblasts
    Lu, David
    Yokoyama, Utako
    Insel, Paul A.
    FASEB JOURNAL, 2008, 22
  • [32] Quantitative studies on liver fibrosis and alpha-smooth muscle actin expression in heroin abusers
    DeAraujo, MST
    Guerret, S
    Gerard, F
    Chossegros, P
    Chevallier, M
    Grimaud, JA
    CELLULAR AND MOLECULAR BIOLOGY, 1997, 43 (04) : 589 - 596
  • [33] Relationship between alpha-smooth muscle actin expression and fibrotic changes in human kidney
    Boukhalfa, G
    Desmouliere, A
    Rondeau, E
    Gabbiani, G
    Sraer, JD
    EXPERIMENTAL NEPHROLOGY, 1996, 4 (04): : 241 - 247
  • [34] Alpha-smooth muscle actin expression by fibroblasts is not a marker or hypertrophic scars in vitro.
    Gerlini, G
    Prignano, F
    Pimpinelli, N
    Borgognoni, L
    Reali, UM
    Romagnoli, P
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1998, 110 (04) : 601 - 601
  • [35] Alpha-smooth muscle actin expression and structure integrity in chondrogenesis of human mesenchymal stem cells
    Shih-Chieh Hung
    Pei-Yin Kuo
    Ching-Fang Chang
    Tain-Hsiung Chen
    Larry Low-Tone Ho
    Cell and Tissue Research, 2006, 324 : 457 - 466
  • [36] INDUCTION OF ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION IN BRAIN PERICYTES BY TGF-BETA-1
    DEWAAL, RMW
    VERBEEK, MM
    OTTEHOLLER, I
    WESSELING, P
    RUITER, DJ
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, : 327 - 327
  • [37] RAT ALVEOLAR MYOFIBROBLASTS ACQUIRE ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION DURING BLEOMYCIN-INDUCED PULMONARY FIBROSIS
    VYALOV, SL
    GABBIANI, G
    KAPANCI, Y
    AMERICAN JOURNAL OF PATHOLOGY, 1993, 143 (06): : 1754 - 1765
  • [38] VENTROLATERAL REGIONALIZATION OF XENOPUS-LAEVIS MESODERM IS CHARACTERIZED BY THE EXPRESSION OF ALPHA-SMOOTH MUSCLE ACTIN
    SAINTJEANNET, JP
    LEVI, G
    GIRAULT, JM
    KOTELIANSKY, V
    THIERY, JP
    DEVELOPMENT, 1992, 115 (04): : 1165 - 1173
  • [39] Immunohistochemical expression of alpha-smooth muscle actin in infiltrating ductal carcinoma of the breast with productive fibrosis
    Tamiolakis, D
    Papadopoulos, N
    Cheva, A
    Lambropoulou, M
    Kotini, A
    Jivannakis, T
    Simopoulos, C
    EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY, 2002, 23 (05) : 469 - 471
  • [40] Mesangial expression of alpha-smooth muscle actin is an early marker for progression of IGA nephropathy.
    Utsunomiya, Y
    Kawamura, T
    Imai, H
    Abe, A
    Hosoya, T
    Sakai, O
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 1997, 8 : A2540 - A2540