ONSET OF EXPRESSION AND REGIONAL DEPOSITION OF ALPHA-SMOOTH AND SARCOMERIC ACTIN DURING AVIAN HEART DEVELOPMENT

被引:54
|
作者
SUGI, Y
LOUGH, J
机构
[1] Department of Cell Biology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin
关键词
SMOOTH MUSCLE ALPHA-ACTIN; SARCOMERIC ALPHA-ACTIN; EARLY HEART DEVELOPMENT; IMMUNOCYTOCHEMISTRY;
D O I
10.1002/aja.1001930203
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The sequential appearance of mRNAs for smooth, cardiac, and skeletal alpha-actin has been described during development of the chicken heart (Ruzicka, D.L., and R.J. Schwartz 1988 J. Cell Biol., 107.2575-2586). To assess whether this reflects the deposition of corresponding isoproteins, we have immunocytochemically localized smooth and sarcomeric (cardiac and skeletal) alpha-actin in Hamburger-Hamilton (H-H) stage 7-18 embryos using monoclonal antibodies. Within the developing embryo at stage 9-, smooth muscle alpha-actin was exclusively detected in the developing heart, upon fusion of the endocardial tubes; sarcomeric alpha-actin was observed later (stage 9). By the onset of contraction at stage 10+, intense immunostaining of both smooth and sarcomeric isoproteins was observed in the ventricle; at this time smooth muscle alpha-actin was also detected in splanchnic mesoderm of the pre-vitelline area, in a cellular layer adjacent to the only embryonic cells that exhibited factor VIII (von Willebrand factor) antigens. Double immunostaining of the myocardium at stage 11, at which time striations were first detected, revealed the co-existence of smooth and sarcomeric actin in developing sarcomeres. Intense expression of sarcomeric actin continued in the heart after stage 11, whereas smooth muscle alpha-actin was down-regulated in the ventricle and became regionalized to the inflow and outflow tracts. As expected, smooth muscle alpha-actin was detected around intra- and extra-embryonic vascular structures at later developmental stages, while sarcomeric actin was observed in somites.
引用
收藏
页码:116 / 124
页数:9
相关论文
共 50 条
  • [11] Alpha-smooth muscle actin expression enhances cell traction force
    Chen, Jianxin
    Li, Hongxia
    SundarRaj, Nirmala
    Wang, James H. -C.
    CELL MOTILITY AND THE CYTOSKELETON, 2007, 64 (04): : 248 - 257
  • [12] Alpha-smooth muscle actin expression upregulates fibroblast contractile activity
    Hinz, B
    Celetta, G
    Tomasek, JJ
    Gabbiani, G
    Chaponnier, C
    MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (09) : 2730 - 2741
  • [13] ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION IN DEVELOPING AND ADULT HUMAN LUNG
    LESLIE, KO
    MITCHELL, JJ
    WOODCOCKMITCHELL, JL
    LOW, RB
    DIFFERENTIATION, 1990, 44 (02) : 143 - 149
  • [14] DEPENDENCE OF COLLAGEN REMODELING ON ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION BY FIBROBLASTS
    ARORA, PD
    MCCULLOCH, CAG
    JOURNAL OF DENTAL RESEARCH, 1994, 73 : 209 - 209
  • [15] EXPRESSION OF ALPHA-SMOOTH MUSCLE (ALPHA-SM) ACTIN DURING CORNEAL STROMAL WOUND-HEALING
    JESTER, JV
    PETROLL, WM
    BARRY, PA
    CAVANAGH, HD
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1995, 36 (05) : 809 - 819
  • [16] ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION IN CULTURED CARDIAC FIBROBLASTS OF NEWBORN RAT
    BROUTYBOYE, D
    KOLONIAS, D
    SAVARAJ, N
    LAMPIDIS, TJ
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 1992, 28A (04) : 293 - 296
  • [17] Lens GSH deficiency mouse Promote Alpha-smooth muscle actin expression
    Wei, Zongbo
    Monnier, Vincent M.
    Fan, Xingjun
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2016, 57 (12)
  • [18] CORRELATION OF ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION AND CONTRACTION IN DUPUYTRENS DISEASE FIBROBLASTS
    TOMASEK, J
    RAYAN, GM
    JOURNAL OF HAND SURGERY-AMERICAN VOLUME, 1995, 20A (03): : 450 - 455
  • [19] Expression of alpha-smooth muscle actin during wound healing in UVR-exposed rabbit corneas
    Podskochy, A
    Koulikovska, M
    Fagerholm, P
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2003, 44 : U247 - U247
  • [20] Temporal expression of alpha-smooth muscle actin and drebrin in septal interstitial cells during alveolar maturation
    Yamada, M
    Kurihara, H
    Kinoshita, K
    Sakai, T
    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2005, 53 (06) : 735 - 744