QUANTITATIVE MAPPING OF INTRACELLULAR CATIONS IN THE HUMAN AMNIOTIC MEMBRANE

被引:11
作者
MORETTO, P [1 ]
LLABADOR, Y [1 ]
SIMONOFF, M [1 ]
RAZAFINDRABE, L [1 ]
BARA, M [1 ]
GUIETBARA, A [1 ]
机构
[1] UNIV PARIS 06,BIOL REPROD LAB,F-75230 PARIS 05,FRANCE
关键词
D O I
10.1016/0168-583X(93)95555-J
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
The effect of magnesium and taurine on the permeability of cell membranes to monovalent cations has been investigated using the Bordeaux nuclear microprobe. PIXE and RBS techniques have been used to provide quantitative measurements and ion distributions in the isolated amniotic membrane. This physiological model for cellular exchanges allowed us to reveal the distribution of most elements involved in cellular pathways and the modifications under different experimental conditions of incubation in physiological fluids. The PIXE microanalysis provided an original viewpoint on these mechanisms. Following this first study, the amnion compact lamina was found to play a role which was not, up to now, taken into account in the interpretation of electrophysiological experimentations. The release of some ionic species, such as K+, from the epithelial cells, during immersion in isotonic fluids, could have been hitherto underestimated.
引用
收藏
页码:275 / 281
页数:7
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