BETA(3) INTEGRIN DERIVED PEPTIDE-217-230 INHIBITS FIBRINOGEN BIDING AND PLATELET-AGGREGATION - SIGNIFICANCE OF RGD SEQUENCES AND FIBRINOGEN-A ALPHA-CHAIN

被引：22
作者：
LASZ, EC
MCLANE, MA
TRYBULEC, M
KOWALSKA, MA
KHAN, S
BUDZYNSKI, AZ
NIEWIAROWSKI, S
机构：
[1] TEMPLE UNIV,HLTH SCI CTR,SCH MED,DEPT PHYSIOL,PHILADELPHIA,PA 19140
[2] TEMPLE UNIV,HLTH SCI CTR,SCH MED,DEPT BIOCHEM,PHILADELPHIA,PA 19140
[3] TEMPLE UNIV,HLTH SCI CTR,SCH MED,SOL SHERRY THROMBOSIS RES CTR,PHILADELPHIA,PA 19140
[4] WISTAR INST,PHILADELPHIA,PA 19104
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 190卷 / 01期
关键词：
D O I：
10.1006/bbrc.1993.1019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
β3 integrin derived peptides 217-230 (DAPEGGFDAIMQAT) and 217-231(Y) (DAPEGGFDAIMQATVY) at 100 μM inhibited 125I-fibrinogen binding to ADP-stimulated platelets and platelet aggregation. Peptide 217-231(Y) (100 μM) significantly inhibited the binding of 125I-albolabrin (a disintegrin with a single RGD sequence) to ADP and thrombin-activated platelets while it had only a slight effect on albolabrin binding to resting platelets. The 125I-β3 217-231(Y) cross-linked selectively to the fibrinogen Aα-chain. The interaction of the RGD sequence in the Aα-chain of fibrinogen with β3 217-231 sequence appears to play a significant role in the events leading to platelet aggregation. © 1993 Academic Press, Inc.
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页码：118 / 124
页数：7
相关论文
共 22 条
[1]  
ANDRIEUX A, 1991, J BIOL CHEM, V266, P14202
[2]  
ANDRIEUX A, 1989, J BIOL CHEM, V264, P9258
[3]   ASSIGNMENT OF DISULFIDE BONDS IN HUMAN PLATELET GPIIIA - A DISULFIDE PATTERN FOR THE BETA-SUBUNITS OF THE INTEGRIN FAMILY [J].
CALVETE, JJ ;
HENSCHEN, A ;
GONZALEZRODRIGUEZ, J .
BIOCHEMICAL JOURNAL, 1991, 274 :63-71
[4]  
CHARO IF, 1991, J BIOL CHEM, V266, P1415
[5]   BINDING OF GLYCOPROTEIN-IIIA-DERIVED PEPTIDE-217-231 TO FIBRINOGEN AND VONWILLEBRAND-FACTORS AND ITS INHIBITION BY PLATELET GLYCOPROTEIN-IIB/IIIA COMPLEX [J].
COOK, JJ ;
TRYBULEC, M ;
LASZ, EC ;
KHAN, S ;
NIEWIAROWSKI, S .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1119 (03) :312-321
[6]   PLATELET GLYCOPROTEIN-IIB-IIIA PROTEIN ANTAGONISTS FROM SNAKE-VENOMS - EVIDENCE FOR A FAMILY OF PLATELET-AGGREGATION INHIBITORS [J].
DENNIS, MS ;
HENZEL, WJ ;
PITTI, RM ;
LIPARI, MT ;
NAPIER, MA ;
DEISHER, TA ;
BUNTING, S ;
LAZARUS, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2471-2475
[7]   LOCALIZATION OF AN ARG-GLY-ASP RECOGNITION SITE WITHIN AN INTEGRIN ADHESION RECEPTOR [J].
DSOUZA, SE ;
GINSBERG, MH ;
BURKE, TA ;
LAM, SCT ;
PLOW, EF .
SCIENCE, 1988, 242 (4875) :91-93
[8]  
DSOUZA SE, 1990, J BIOL CHEM, V265, P3440
[9]  
FARRELL DH, 1992, IN PRESS P NATL ACAD
[10]  
GOULD RJ, 1990, P SOC EXP BIOL MED, V195, P168, DOI 10.3181/00379727-195-43129B
123