High molecular weight DNA from nucleated erythrocytes for use in pulsed-field gel electrophoresis (PFGE)

被引：1

作者：

VenturaHolman, T ^{[1
]}

Lobb, CJ ^{[1
]}

机构：

[1] UNIV MISSISSIPPI,MED CTR,DEPT MICROBIOL,JACKSON,MS 39216

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1995年 / 12卷 / 02期

关键词：

pulsed-field gel electrophoresis; erythrocytes; DNA; hemoglobin;

D O I：

10.1016/1050-3862(96)88053-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleated erythrocytes of lower vertebrates provide a source of genomic DNA that can be used in pulsed-field gel electrophoresis (PFGE) studies. Difficulties reported in the preparation of chicken erythrocyte DNA for analysis by PFGE suggest that the presence of hemoglobin iron may result in iron-mediated DNA degradation. We report here modifications to the procedures established for isolation of high molecular weight DNA from mammalian cells. By increasing the volume of buffers and extending the incubation periods to allow for the removal of hemoglobin iron, we have successfully prepared channel catfish erythrocyte DNA suitable for analysis by PFGE.

引用

页码：101 / 103

页数：3

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