NUCLEOTIDE AND PREDICTED AMINO-ACID-SEQUENCE OF A CDNA CLONE ENCODING PART OF HUMAN TRANSKETOLASE

被引：22

作者：

ABEDINIA, M ^{[1
]}

LAYFIELD, R ^{[1
]}

JONES, SM ^{[1
]}

NIXON, PF ^{[1
]}

MATTICK, JS ^{[1
]}

机构：

[1] UNIV QUEENSLAND,DEPT BIOCHEM,CTR MOLEC BIOL & BIOTECHNOL,BRISBANE,QLD 4072,AUSTRALIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0006-291X(05)80312-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transketolase is a key enzyme in the pentose-phosphate pathway which has been implicated in the latent human genetic disease, Wernicke-Korsakoff syndrome. Here we report the cloning and partial characterisation of the coding sequences encoding human transketolase from a human brain cDNA library. The library was screened with oligonucleotide probes based on the amino acid sequence of proteolytic fragments of the purified protein. Northern blots showed that the transketolase mRNA is approximately 2.2 kb, close to the minimum expected, of which approximately 60% was represented in the largest cDNA clone. Sequence analysis of the transketolase coding sequences reveals a number of homologies with related enzymes from other species. © 1992 Academic Press, Inc.

引用

页码：1159 / 1166

页数：8

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